101. CRISPR-Cas9 technology is typically used for
1. Genomic Sequencing
2. Gene editing
3. DNA replication
4. Gene Mapping

Correct Answer:

2. Gene Editing

Explanation:

CRISPR-Cas9 is a groundbreaking tool in molecular biology, specifically used for gene editing. Here’s a breakdown of each option:

1. Genomic Sequencing:
   While genomic sequencing helps determine the sequence of nucleotides in DNA, CRISPR-Cas9 is not directly used for sequencing. Sequencing involves reading the DNA, not editing it.

2. Gene Editing:
   CRISPR-Cas9 is a gene-editing technology that allows for precise modifications of DNA at specific locations. This system uses a guide RNA to target a particular gene, and the Cas9 enzyme acts as “molecular scissors” to cut DNA at the desired location. This cutting enables either the addition, removal, or alteration of specific genes.

3. DNA Replication:
   CRISPR-Cas9 does not play a role in DNA replication. DNA replication involves the copying of DNA during cell division, which is a process driven by enzymes like DNA polymerase.

4. Gene Mapping:
   While gene mapping involves determining the position of genes on chromosomes, CRISPR-Cas9 is primarily used for editing genes rather than mapping their locations.

Conclusion:

CRISPR-Cas9 is widely recognized for its application in gene editing, allowing scientists to alter genetic material with precision.

Correct Answer: 2. Gene Editing