Q. 26 To which one of the following classes of enzymes does chymotrypsin belong?
(A) Oxidoreductase
(B) Hydrolase
(C) Transferase
(D) Isomerase

Chymotrypsin Enzyme Class: MCQ Answer and Explanation
Chymotrypsin belongs to the hydrolase class of enzymes. It functions as a serine protease that catalyzes peptide bond hydrolysis in proteins.

Correct Answer
B) Hydrolase​

Chymotrypsin specifically cleaves peptide bonds on the C-terminal side of aromatic amino acids like phenylalanine, tyrosine, and tryptophan. This hydrolysis reaction adds water across the bond, confirming its hydrolase classification.​

Oxidoreductase Explanation
Oxidoreductases catalyze oxidation-reduction reactions involving electron transfer. Chymotrypsin does not perform redox reactions, so this option is incorrect.​

Hydrolase Explanation
Hydrolases break chemical bonds using water, including proteases like chymotrypsin. This matches chymotrypsin’s mechanism of hydrolyzing peptide amide bonds.​

Transferase Explanation
Transferases move functional groups from one molecule to another. Chymotrypsin lacks this transfer activity, making this option wrong.​

Isomerase Explanation
Isomerases rearrange atoms within a molecule to form isomers. Chymotrypsin does not isomerize substrates, ruling out this choice.​