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(FEB 2022-1) 28. The enzyme alkaline phosphatase was tested for its catalytic activity using the substrate para- nitrophenylphosphate. The KM obtained was 10 mM and Vmax was 100umol/min. Which one […]

(JUNE 2004) 26. During an enzyme assay if the initial velocity Vo = 1/2 Vmax, then substrate concentration will be equal to (1) α Km                                                 (2) Km (3) 1/2 Km                                               […]

(FEB 2022-1) 25. The following statements are being made to define the Michaelis constant (KM). It is: A. Independent of enzyme concentration [E] and substrate concentration [S] B. Equal to […]

(JUNE 2007) 24. If the Km of enzyme for substrate A is 1 x 10-6 and for substrate B is 4 x 10-8, it means (1) Enzyme has more affinity […]

The correct answer is (4) B ⟶ C (Enzyme EB). Introduction Multistep biochemical pathways often involve several enzyme-catalyzed reactions, each with unique kinetic properties. The rate-limiting step determines the overall speed […]

The correct answer is (2) B and D. Introduction Michaelis-Menten kinetics is essential for understanding how enzymes catalyze biochemical reactions. Two critical parameters—Km (Michaelis constant) and kcat (turnover number)—describe how efficiently […]

(DEC 2019 ASSAM) 21. Following are statements on enzyme kinetics. Choose the correct statement. (1) Sufficiently high concentrations of substrate cannot completely relieve competitive inhibition. (2) Sufficiently high concentrations of […]

(DEC 2011) 19. Michaelis and Menten derived their equation using which of the following assumption? (1) Rate limiting step in the reaction is the breakdown of ES complex to product […]