Given below is a schematic representation of a Southern blot performed to identify single copy integration events of the T-DNA among six transgenic plants (T1 — T6). Which one of […]
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CSIR NET Life Science Previous Year Questions and Solution on EXTENSION OF MENDELIAN PRINCIPLES (62)
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Using Southern blot with LB/RB probes to detect true single-copy T‑DNA insertions
Given below is a schematic representation of the T-DNA region of a binary vector used for genetic transformation of plants. LB: Left Border, RB: Right Border, M: Marker gene, P: […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Identifying single-copy T-DNA insertions from Southern blot patterns
Given below is a schematic representation of the T- DNA region of a transgene construct and the Southern blot analysis (using Pst l digested genomic DNA) of five independent transgenic […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Using Southern blot patterns to estimate transgene copy number in plants
Genomic DNA of transgenic plants (P1, P2 and P3) obtained by transforming with binary vector A whose map is depicted below, was digested with BamH I and Sal I and […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Interpreting Southern blot patterns to infer T-DNA copy number, truncation and arrangement
A transgenic plant is developed with the following T- DNA construct In order to analyze the nature of integration, genomic DNA digested with EcoRl was used for Southern hybridization using […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
How to distinguish transgenic from non-transgenic plants when the insertion site is unknown
Given below are a few approaches/techniques used in the analysis of plants: A. Phenotype linked to the marker gene. B. PCR using primers specific to the host genome. C. Southern […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Methods to determine copy number and tissue-specific expression in transgenic animals
During transgenesis, the location of the genes and their number integrated into the genome of the transgenic animal are random. It is often necessary to determine the copy number of […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Interpreting selection-agent efficacy and explant suitability from regeneration data
A group of researchers are testing two new agents, M1 and M2 for their efficacy in selecting transgenic plants. When they performed tissue culture experiments using three explants, A, B, […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
How RNA silencing controls plant viruses and the role of P19
RNA silencing is an important strategy to control viral infection in plants. The following statements were made regarding RNA silencing. A. It is driven by small interfering RNA (SiRNA) derived […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Strategies to increase yield of a commercially important enzyme from a natural isolate
In an attempt to increase the yield of a commercially important enzyme from natural isolate several strategies were adopted as follows: (A) Genome was selectively modified to increase yield. (B) […]
