Gene therapy is a promising tool for addressing several diseases in humans. With respect to the above, which one of the following statements is FALSE? (1) Gene therapy involves the […]
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CSIR NET Life Science Previous Year Questions and Solution on EXTENSION OF MENDELIAN PRINCIPLES (62)
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Which human stem cells are actually used in somatic cell gene therapy?
Following are the different ways of obtaining human stem cells- (A) Cells from morula are dispersed and cultured to give rise pluripotent stem cells. (B) Isolated inner cell mass of […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Safest stem‑cell strategy for liver regeneration using patient‑specific iPS cells
Stem cells are widely used for their regenerative property and capacity to differentiate into different lineages. A person with a damaged liver approaches a stem cell therapist. Which of the […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Explaining transient increase and recovery of muscle tone after caudate lesion
The muscle tone was increased after electrolytic lesion of the caudate nucleus in a cat. The muscle tone decreased within seven days. The following explanations were given by the researcher. […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Maintaining a line of heterozygous transgenic mice
Inbreeding for 5 generations led to production of homozygous transgenic mice. However, these homozygous males or females were infertile. Which of the following approach is most preferable and economical to […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Why DMEM turns yellow in liver cell culture
A student added DMEM culture medium which was pink in colour to growing liver cells. Three days later the medium colour was yellow. This indicated (1) change in cell morphology […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Method to detect mycoplasma contamination in mammalian cell cultures
Which is best method for checking mycoplasma contamination in a mammalian cell line? (1) Southern Hybridization (2) ELISA (3) PCR (4) Western Hybridization The best method is (3) PCR. Why PCR […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Reagents used to dissociate primary tissues into single cells
To prepare individual tissue cells from a primary culture, the cell-cell and cell-matrix interaction must be broken. To achieve this would NOT use: (1) EDTA […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Key facts about maintaining mammalian cells in vitro
Which one of the following statements is NOT correct for propagation and maintenance of mammalian cells in vitro? (1) Transformed cell lines do need external supply of serum to grow. […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Procedure to develop a transgenic mouse by pronuclear microinjection
The following statements are made regarding developing a transgenic mouse: A. The transgenic mouse thus born will be a homozygous transgenic animal and can be maintained by crossing with another […]
