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Q.41 Aspartate residues are found in the active sites of many enzymes. The 𝐩𝐊𝐚 for the 𝜷-carboxylate of aspartate is 3.86 . At physiological pH this group can function as […]

Q.40 An enzyme was purified using ion-exchange chromatography and the results are shown in the table below. Step                          […]

Q.39 Three micrograms of a circular plasmid of 4200 bp was digested with a restriction enzyme and subjected to agarose gel electrophoresis. Five DNA fragments of different sizes were observed […]

Q.38 When freshly isolated intact mitochondria were incubated with ADP and inorganic phosphate neither the oxygen consumption nor the ATP synthesis could be detected. Addition of succinate resulted in increased […]

Q.37 The 𝚫𝐆′𝐨for the malate dehydrogenase catalyzed step of Krebs cycle is +𝟕. 𝟏𝐤𝐜𝐚𝐥/𝐦𝐨𝐥𝐞.Nevertheless, the conversion of malate to oxaloacetate in vivo proceeds spontaneously because the subsequent reaction that consumes […]

Q.36 Four groups of metabolites are given below. Choose the group in which all the compounds contain at least one bond whose 𝚫𝐆′𝟎 of hydrolysis is ≤ −𝟕. 𝟎𝐤𝐜𝐚𝐥/𝐦𝐨𝐥𝐞. (A) […]

Q.35 Polypeptides are biosynthesized on the ribosomes inside the cell. Chemical synthesis of polypeptides is also possible through Merrifield’s solid-phase peptide synthesis. In both the cases the polypeptide chain is […]

Q.34 The fluidity of a phospholipid membrane increases when the fatty acid (A) chain length increases and degree of unsaturation decreases (B) chain length decreases and degree of unsaturation increases […]

Q.33 Molar absorption spectra labeled (i), (ii) and (iii) for three different amino acids are shown below. Which one of the following is the correct combination of spectral assignments? (A) […]

Q.32 Cellulose serves as a structural polymer whereas starch does not. This is because cellulose contains (A) 𝛽1 → 4 linked glucose monomers and inter-chain hydrogen bonds (B) 𝛽1 → […]