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Q.34 Consider the following biochemical reaction:Fructose 6–phosphate + ATP Fructose 1,6–bisphosphate + ADPThe equilibrium constant under biochemical standard conditions (K’eq) for the above reaction is 254.The standard free energy change […]

Q.33 For a single substrate enzyme, a reaction is carried out at a substrate concentration four times thevalue of Km. The observed initial velocity will be _________ % of Vmax. […]

Q.32 Which one of the following molecules is an allosteric activator of phosphofructokinase–1?(A) Fructose 1,6–bisphosphate (B) Fructose 2,6–bisphosphate(C) Glucose 6–phosphate (D) Citrate Answer: (B) Fructose 2,6-bisphosphate Fructose 2,6-bisphosphate (F2,6BP) is the […]

Q.31 The affinity of an antibody can be determined quantitatively by(A) MALDI–TOF MS (B) isoelectric focusing(C) SDS–PAGE (D) equilibrium dialysis Answer: (D) equilibrium dialysis Equilibrium dialysis quantitatively measures antibody affinity (Kd) […]

Q.30 Choose the option with two reducing sugars.(A) Lactose and Maltose (B) Trehalose and Sucrose(C) Maltose and Trehalose (D) Lactose and Sucrose Answer: (A) Lactose and Maltose Reducing sugars possess a […]

Q.29 The receptor for epinephrine is a(A) Tyrosine kinase receptor (B) Serine–threonine kinase receptor(C) G–protein–coupled receptor (D) Ligand activated transcription factor Answer: (C) G-protein-coupled receptor Epinephrine (adrenaline) binds to adrenergic receptors […]

Q.28 Which one of the following proteases does NOT cleave on the carboxyl side of any Arginineresidue in a protein?(A) Trypsin (B) Proteinase K(C) Thrombin (D) Chymotrypsin Answer: (B) Proteinase […]

Q.27 A mixture of Arginine, Phenylalanine and Histidine was fractionated using cation exchangechromatography at neutral pH. The amino acids were eluted with an increasing salt gradient. Identifythe correct order of […]

Q.26 During an enzyme catalyzed reaction, the equilibrium constant(A) increases(B) decreases(C) remains unchanged(D) can increase or decrease, depending on the enzyme Answer: (C) remains unchanged Enzymes act as catalysts that speed […]

Q.25 The order of acidity of the following acids is Given Compounds Compound Substituent (1) –OCH3 (Methoxy) (2) –CH3 (Methyl) (3) –NO2 (Nitro) (4) –H (Benzoic acid) Key Concept: Acidity […]