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59. In which type of chromatography are ion-pairing agents used for elution? A. Hydrophobic interaction chromatography B. Reverse phase chromatography C. Ion exchange chromatography D. Immobilized metal anion chromatography Answer: […]

58. One of these purification steps requires initial high ionic strength in the sample: A. Ion exchange chromatography B. Hydrophobic interaction chromatography C. Chromatofocusing D. Preparative chromatography Answer: (B) Hydrophobic […]

57. Carboxymethyl cellulose is: A. A cation-exchange matrix B. A gel filtration matrix C. An anion-exchange matrix D. A plant cell wall constituent Answer: (A) A cation-exchange matrix Carboxymethyl cellulose […]

56. Affinity chromatography is a method that can be used to purify cell surface receptors, while they retain their hormone binding ability. A ligand (hormone) for a receptor of interest […]

55. Two monomeric proteins migrate identically in SDS-polyacrylamide gel electrophoresis, but get separated in a gel-permeation chromatography experiment. This could be because: A. They have different isoelectric points B. One […]

54. Which would be best to separate a protein that binds strongly to its substrate? A. Gel filtration B. Affinity chromatography C. Cation exchange D. Anion exchange Correct Answer: B. […]

53. The relative solubility of solute in both the phases determines the: A. Rate of movement of solvent B. Rate of disappearance of solvent C. Rate of movement of solute […]

52. A proper solvent that is passed through the column for elution so that separated components can be collected is called: A. Adsorbent B. Buffer solution C. Mobile phase D. […]

51. The gas-solid chromatography is __________ chromatography as per basic principle involved. A. Exclusion B. Ion-exchange C. Adsorption D. Absorption Correct Answer: C. Adsorption Gas-solid chromatography separates analytes through physical […]

50. In size exclusion chromatography, solute molecules are separated based on: A. Molecular geometry and size B. Molecular composition C. Molecular phase D. Molecular formula Correct Answer: A. Molecular geometry […]