24. The most important property of any microscope is its resolution (D) and can be calculated from the formula: D = 0.61λ / (µ sin θ) where D is the minimum distance […]
23. A microorganism when viewed under a compound microscope with objective of 10× magnification measured 400 nm in length. The same microorganism when viewed under a dissecting microscope with 10× lens […]
22. In resting cells, proteins X and Y are localized in the cytosol. Upon stimulation with lipopolysaccharide (LPS), both of them are phosphorylated and translocate to the nucleus. You have […]
21. Co-localization of two fluorescently labelled proteins in an organelle in cells is usually visualized by: A. Interference-contrast microscopy B. Scanning electron microscopy C. Confocal microscopy D. Atomic force microscopy […]
20. Match the following microscopic techniques with their respective functions Microscopic technique Functions 1. Phase contrast microscopy 2. Fluorescent microscopy 3. Transmission electron microscopy 4. Bright field microscopy 5. Dark […]
19. In scanning electron microscope (SEM) to form an image of the specimen, (A) An electron should pass through the specimen (B) Electrons are scattered from the surface of the […]
18. The movement of proteins within the nucleus can be determined by: A. Electron microscopy B. Fluorescence recovery after photobleaching C. Fluorescent in situ hybridization D. Confocal light microscopy Fluorescence […]
17. You wish to localize a given gene product at subcellular levels following immunofluorescence staining. Routine microscopy could not resolve whether the gene product is localized inside the nucleus or […]
16. The movement of a single cell was required to be continually monitored during development. This cell was marked with a reporter gene. To visualize this movement one would use: […]
15. Bindin is a protein that is expressed in the tip of the sperm head (acrosome) and is important for sperm–egg interaction. Its distribution pattern changes during sperm maturation. Which […]
