Q.45 Match List-I with List-II
| List-I (Technique) | List-II (Connecting / Purpose) |
|---|---|
| (A) DNA footprinting | (I) Protein–protein interaction |
| (B) Yeast two hybrid system | (II) VNTR |
| (C) DNA fingerprinting | (III) DNA binding protein |
| (D) SAGE | (IV) Transcriptome analysis |
Choose the correct answer from the options given below:
- (A) – (I), (B) – (II), (C) – (IV), (D) – (III)
- (A) – (III), (B) – (I), (C) – (II), (D) – (IV)
- (A) – (III), (B) – (IV), (C) – (I), (D) – (II)
- (A) – (IV), (B) – (II), (C) – (I), (D) – (III)
DNA Footprinting to SAGE: Matching Molecular Biology Techniques – Correct Answer Revealed
DNA footprinting, yeast two-hybrid, DNA fingerprinting, and SAGE are key techniques in molecular biology exams like CUET PG. The correct matching pairs each with its primary purpose for accurate exam prep.
Correct Answer
The right option is (B) (A) – (III), (B) – (I), (C) – (II), (D) – (IV). This links DNA footprinting to DNA binding proteins, yeast two-hybrid to protein-protein interactions, DNA fingerprinting to VNTR, and SAGE to transcriptome analysis.
Technique Matches Explained
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(A) DNA footprinting – (III) DNA binding protein: DNA footprinting uses DNase I to cleave unprotected DNA, leaving “footprints” where proteins bind and shield specific sequences.
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(B) Yeast two-hybrid system – (I) Protein–protein interaction: This system detects interactions by fusing proteins to transcription factors in yeast, activating reporters if they bind.
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(C) DNA fingerprinting – (II) VNTR: It analyzes Variable Number Tandem Repeats (VNTR) polymorphisms for unique genetic profiles in forensics.
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(D) SAGE – (IV) Transcriptome analysis: Serial Analysis of Gene Expression (SAGE) sequences short tags from mRNA to quantify gene expression genome-wide.
Why Other Options Fail
Option (A) mismatches all pairs, like linking DNA footprinting to protein-protein interactions.
Option (C) wrongly ties footprinting to transcriptome work and SAGE to VNTR.
Option (D) incorrectly assigns footprinting to SAGE’s role and others swapped.Exam Tips
Focus on core principles: protection assays for binding sites, reporter activation for interactions, repeat variability for IDs, and tag sequencing for transcripts. Practice similar match-ups in genetics and bioinformatics sections.
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