Southern Blotting: The Best Technique for DNA Detection
Which blotting technique is used for the detection of DNA that has been separated from a mixture of DNA restriction fragments by electrophoresis through an agarose gel and then transferred onto a nitrocellulose sheet ?
A. Eastern blotting
B. Northern bloting
C. Sourthern blotting
D. Westem blotting
Which Blotting Technique Detects DNA?
Blotting techniques are used in molecular biology to detect specific biomolecules like DNA, RNA, or proteins. Among them, Southern blotting is specifically designed for DNA analysis.
The correct answer is:
✅ C. Southern Blotting
What Is Southern Blotting?
Southern blotting is a technique developed by Edward Southern in 1975 to identify specific DNA sequences in a complex mixture.
How Does Southern Blotting Work?
-
Restriction Enzyme Digestion
- DNA is cut into fragments using restriction enzymes.
-
Agarose Gel Electrophoresis
- The digested DNA fragments are separated by size using agarose gel electrophoresis.
-
Transfer to Nitrocellulose or Nylon Membrane
- The DNA is transferred from the gel onto a nitrocellulose or nylon membrane via capillary action.
-
Hybridization with a DNA Probe
- A radioactive or fluorescent DNA probe is used to bind to the specific target DNA sequence.
-
Detection
- The probe-labeled DNA is detected using autoradiography or chemiluminescence.
Why the Other Options Are Incorrect
❌ A. Eastern Blotting
- Used to analyze post-translational modifications of proteins, not DNA.
❌ B. Northern Blotting
- Detects RNA, not DNA.
❌ D. Western Blotting
- Used for protein detection, not DNA.
Conclusion
Southern blotting is the correct technique for detecting DNA fragments that have been separated by agarose gel electrophoresis and transferred onto a nitrocellulose membrane. It is widely used in gene mapping, genetic fingerprinting, and diagnosing genetic disorders.
91 Comments
Suman bhakar
March 3, 2025Ok sir
Mahima Sharma
August 21, 2025Southern blotting
Ujjwal
March 3, 2025👍✔️
Muskan singodiya
August 21, 2025Southern blotting
Akshay mahawar
March 3, 2025Done👍
Arushi
March 3, 2025👍
Arushi
March 3, 2025👍👍
Jyoti Meena
March 3, 2025Done
Abhilasha
March 3, 2025Right ✅
Priyam choudhary
March 3, 2025👍
Priya khandal
March 3, 2025Right sir
Nisha
March 4, 2025Right
Jonu Meena
March 4, 2025👍🏻👍🏻
Prami Masih
March 4, 2025Done sir
Lokesh kumawat
March 4, 2025Done
VIKRAM GAHLOT
March 7, 2025ok sir
Meenakshi Choudhary
August 19, 2025Sourthern blotting
Khushi Vaishnav
August 19, 2025Southern Blotting
Neelam Sharma
August 19, 2025Southern Blotting
Aakansha Sharma
August 20, 2025Southern blotting
Dipti Sharma
August 20, 2025The correct answer is
C. Southern Blotting
Khushi Mehra
August 20, 2025Southern blotting is the correct answer for detection of specific DNA
shruti sharma
August 20, 2025southern blotting
Diksha Chhipa
August 20, 2025Southern blotting used for dna
Mohd juber Ali
August 20, 2025C is right
Anurag Giri
August 20, 2025Ans c
Southern blotting
Dharmpal Swami
August 20, 2025Southern blotting -specific dna sequence
Bharti Yadav
August 20, 2025Southern blotting – detect specific dna sequence.
Nilofar Khan
August 20, 2025Southern blotting
Mansukh Kapoor
August 20, 2025The correct answer is option 3rd
Southern blotting
Varsha Tatla
August 20, 2025Southern blotting
Sakshi yadav
August 20, 2025Southern blotting
Aman Choudhary
August 20, 2025Option C southern blotting
Priya choudhary
August 20, 2025Correct answer is option c, southern blotting
Surbhi Rajawat
August 20, 2025Southern blotting is a technique to identify a specific DNA sequence from many fragments cut by REN.
Priya dhakad
August 20, 2025Southern blotting is used for detection of specific DNA
Deepika Sheoran
August 20, 2025Option c is correct Southern blotting.
anjani sharma
August 20, 2025Southern blotting
Aafreen
August 20, 2025Ans 3- southern blotting
Kavita choudhary
August 20, 2025Sourthan blotting for DNA
Manisha
August 21, 2025Separation dna from mixture electrophoresis is through an agrose gel then transfer on the cellulose sheet *- southern blotting
Neha
August 21, 2025Southern blotting used to detect dna
Payal Gaur
August 21, 2025Southern blotting technique
Sneha .......
August 21, 2025Surthern blotting
Mitali saini
August 21, 2025✅ C. Southern Blotting
Santosh Saini
August 21, 2025In Southern blotting agarose gel electrophoresis used for separation of DNA
Khushi Pareek
August 21, 2025Southern blotting
Sheikh Aleen
August 21, 2025Southern blotting is the correct technique for detecting DNA fragments.
Meera Gurjar
August 21, 2025Southern blotting
Simran Saini
August 21, 2025Southern blotting.
Avni
August 21, 2025Southern Blotting is the Best Technique for DNA Detection
Bhawna Choudhary
August 21, 2025Southern blotting
Devika
August 21, 2025Southern blotting
Divya rani
August 21, 2025Southern blotting is technique to detect the specific dna sequence and we use nitrocellulose membrane
Sakshi Kanwar
August 22, 2025Southern blotting
Soniya Shekhawat
August 22, 2025Southern blotting
karishma
August 22, 2025southern blotting is technique to detect the specific dna sequence and transfer on nitrocellulose membrane
Arushi
August 22, 2025Southern blotting
Kirti agarwal
August 23, 2025Southern blotting
Priya khandal
August 23, 2025Southern blotting
Ankita Pareek
August 23, 2025Southern blotting
Akshita Jangid
August 23, 2025Southern Blotting
Devika
August 24, 2025Southern blotting used for dna detection
Yogita
August 24, 2025Southern blotting
Himanshi
August 24, 2025Southern blotting
Anisha Beniwal
August 24, 2025For DNA detection southern blotting is done
AKANKSHA RAJPUT
August 24, 2025Southern blotting
Shivani
August 24, 2025Southern blotting is used to detect specific DNA sequence
Bhavana kankhedia
August 24, 2025Southern Blotting
Ayush
August 24, 2025Southern blotting
Kajal
August 24, 2025Southern blotting is correct one
Tanvi Panwar
August 24, 2025Southern blotting is used for the detection of specific DNA.
Priyanka Verma
August 24, 2025Southern blotting
Sonam Saini
August 24, 2025Southern bloating is used for the detection of specific DNA
Surendra Doodi
August 24, 2025Southern bloating is used for the detection of specific DNA…..
sakshi khatri
August 24, 2025southern blotting
Falguni Prakash
August 24, 2025Southern blotting for specific dna sequence
Swati Choudhary
August 24, 2025done
Komal Sharma
August 25, 2025Southern blotting is the correct technique for detecting DNA fragments that have been separated by agarose gel electrophoresis and transferred onto a nitrocellulose membrane. It is widely used in gene mapping, genetic fingerprinting, and diagnosing genetic disorders.
Kajal
August 25, 2025Southern blotting
Priyanka Meena
August 26, 2025Southern blotting
Muskan Yadav
August 26, 2025Southern blotting
Manisha choudhary
August 27, 2025Southern blotting correct answer h southern blotting m DNA ko restriction endonuclease enzyme s fragments kiye jaate h fir agarose gel p run krwaya jata h the DNA probe diya jata h then chemiluminance ya autoradiography k dwara detection kiya jata h
Estern blotting m post translation modification pta krte h
Northern blotting rna k liye hoti h
Western blotting protein k liye hoti h
Kanica Sunwalka
August 28, 2025done
Shriyanshi verma
August 30, 2025Southern Blotting
Alec
August 30, 2025correct ans. is southern blotting.
Asha Gurzzar
August 30, 2025Southern blotting
Pratibha Jain
August 30, 2025correct answer is option (C) Southern Blotting
Anisha jakhar
August 31, 2025Southern blotting
Shobha Kanwar
August 31, 2025Southern blotting is the correct answer for detection of specific DNA
Deepika sheoran
November 15, 2025Southern blotting