A protein in 100 mM KCl solution was heated and the observed Tm (midpoint of unfolding) was 600C. When the same protein solution in 500 mM KCl was heated, the […]
Tag: Stability of Proteins PYQ
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Protein Denaturation by Organic Solvents: Hydrophobic Interactions Are Mainly Affected
Globular protein when treated with organic solvent get denatured. The main interaction which is affected on treatment with organic solvent is (1) Hydrogen bonds (2) Covalent bonds (3) Ionic interactions […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
What Determines the Shape of Protein Stability Curves? The Crucial Role of Hydrophobic Interactions
Values of Tm (midpoint of denaturation), ΔHm (enthalpy change at Tm) and ΔCP (constant-pressure heat capacity change) of a protein are measured in a differential scanning calorimeter. ΔGD(T), the Gibbs […]
CSIR NET Previous Year Questions and Solutions on Stability of Proteins and Nucleic Acids
How Glycerol Stabilizes Solutions of Proteins
Glycerol is added to protein solutions to stabilize the preparations by (1) increasing the viscosity of solution (2) stabilizing the pH (3) preferential hydration of proteins (4) interacting and neutralising […]
