Why E. coli DNA Polymerase Cannot Replace Taq Polymerase in PCR If E. coli DNA polymerase instead of Taq Polymerase is used in a classical PCR-reaction,A researcher will have to […]
Tag: Polymerase Chain Reaction
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CSIR NET Previous Year Questions on PCR
Identifying Radiolabeled PCR Products Based on dNTP Labeling
Identifying Radiolabeled PCR Products Based on dNTP Labeling A researcher perform 4 reactions of PCR which are identical babbled (ABCD) by usual mix of dNTPs, each of tube contained γ-32P […]
CSIR NET Previous Year Questions on PCR
TaqMan Assay for Genotyping and Base Substitution Detection
TaqMan Assay for Genotyping and Base Substitution Detection In ‘TagMan’ assay for detection of base substitutions (DNA variant), probes (oligonucleotides) with Fluorescent dyes at the 5′-end and a quencher at […]
CSIR NET Previous Year Questions on PCR
Detection of Fluorescence in Real-Time PCR Using Molecular Beacons and Taqman Probes
Detection of Fluorescence in Real-Time PCR Using Molecular Beacons and Taqman Probes Molecular beacons (MB) and Taqman (TQ) are used as probes in Real time PCR experiments. Both these probes […]
CSIR NET Previous Year Questions on PCR
How to Test Translational Block in Recombinant Protein Expression
Best Technique to Test Translational Block in Recombinant Protein Expression A recombinant protein is found to be expressed very poorly in E.coli. It is hypothesized that the expression is blocked […]
CSIR NET Previous Year Questions on PCR
Highest Resolution Technique for Chromosomal Alterations
Highest Resolution Technique for Detecting Chromosomal Alterations Which one of the following techniques is of highest resolution for detection of chromosomal alterations? A. PCR B. CGH C. G-banding D. C-banding […]
CSIR NET Previous Year Questions on PCR
Essential Components Required for PCR Amplification
2Essential Components Required for PCR Amplification DNA from a host sample can be amplified by a process known as the polymerase chain reaction (PCR). Which of the following is required […]
CSIR NET Previous Year Questions on PCR
The Differences Between PCR, Branched DNA & Ligase Chain Reaction
Understanding the Differences Between PCR, Branched DNA, and Ligase Chain Reaction Which one of the following statements is not correct? A. PCR is more sensitive than branched DNA assays B. […]
CSIR NET Previous Year Questions on PCR
Tryptophan and Methionine Codons as PCR Primers
Why Investigators Prefer DNA Sequences Rich in Tryptophan and Methionine Codons for PCR Primers In selecting a DNA sequence to be used as a template for construction of PCR primers, […]
CSIR NET Previous Year Questions on PCR
Do Parent DNA Strands Recombine During PCR Annealing
Why Do Parent DNA Strands Not Recombine During PCR Primer Annealing? During the polymerase chain reaction, the two DNA strand are separated at 95 °C after which the reaction mixture […]
