In order to identify the regulatory regions of a novel promoter sequence shown above, four 150 bp deletion constructs were made in a luciferase reporter system as indicated above in […]
Tag: molecular technique
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CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Promoter Deletion Analysis: Identifying Activator and Repressor Binding Sites in Gene Regulation
3. A promoter deletion study was done in order to determine the binding sites for a transcription factor on the promoter, which is activated on treatment with the drug ‘X’. […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Co-immunoprecipitation: The Most Effective Method to Study Protein-Protein Interactions
Which of the following methods can be used to study protein-protein interactions? (1) Co-immunoprecipitation (2) Peptide mapping (3) Radiolabeling (4) Enzyme linked immunoassay Introduction Protein-protein interactions (PPIs) are fundamental to […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
DNase I Foot printing to Study Transcription Factors and Their Binding Sites
1. Which technique is most suitable to study transcription factor and its binding site? (1) DNAse I foot printing (2) Western blotting (3) Northern blotting (4) Microarray Introduction Understanding […]
