Reverse transcriptase of retero virus is- (1) DNA dependent DNA polymerase (2) DNA dependent RNA polymerase (3) RNA dependent DNA polymerase (4) RNA dependent RNA polymerase What Is Retroviral […]
Tag: molecular technique
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CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Topoisomerases as Targets for Anticancer Drugs: Clarifying Common Misconceptions
As topoisomerases play an important role during replication, a large number of anticancer drugs have been developed that inhibit the activity of these enzymes. Which of the following statements is […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How DNA Polymerase I Synthesizes DNA on Different DNA Substrates: Single-Stranded, Nicked, Linear, and Circular Molecules
In a cell free extract containing DNA polymerase I, Mg2+, dATP, dGTP, dCTP and dTTP3H), the following DNA molecules were added: Single stranded closed circular DNA moleculecontaining 824 nucleotides. Single […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Autoradiography Patterns In Vitro Translation with Cyanogen Bromide Cleavage
11. An in vitro translation system capable of incorporating ~8 amino acids was programmed to translate a single mRNA that codes for an alanine-rich (35% alanine with uniform distribution of […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Chromatin Immunoprecipitation (ChIP) Assay
10. A reporter cell line with stably integrated retroviral promoter-luciferase construct was transfected with an expression vector for a cellular protein. The protein seems to regulate the activation of retroviral […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
How to Identify Enhancer Sequences Using Reporter Constructs
9. A researcher wanted to identify the enhancer sequences of a newly discovered gene. Shown below are the relevant regions of some of the reporter constructs the researcher designed to […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
S1 Nuclease Mapping: Understanding Transcript Length and Start Sites
The data from an S1 nuclease mapping experiment for a transcript mfg1 using a 5′-end labelled probe are shown below. Following interpretations were made: A. The liver RNA is 500 […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Interpreting Run-Off Transcription Assay Results: Specificity of Novel Sigma Factors for Promoters
7. Run off transcription assays were performed to establish the specificity of three novel sigma factors for their promoters. Result of the experiments are shown below: Following inferences were made […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Interpreting EMSA Results: Understanding Protein-DNA and Protein-Protein Interactions in Gene Regulation
6. A researcher was working with three proteins, A, B and C which may have potential roles in gene expression. In order to validate the hypothesis, EMSA (electrophoretic mobility shift […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Promoter Deletion Analysis To Identify Negative Regulatory Element
Deletion analysis of a promoter region of a gene was carried out to identify the regulatory elements in it. In the figure below, the filled boxes denote the areas of […]
