9. You have isolated a hypothetical protein X. When X is run on a gel filtration column, the apparent size of the protein is 80 kDa. When X is run […]
Tag: dbt jrf pyq 2021
Tag: dbt jrf pyq 2021
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How to Mimic Protein Phosphorylation by Mutating Threonine Residue: Exploring the Best Substitution
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- April 13, 2025
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8. A protein X is active when phosphorylated on Thr residue. You wish to mimic this phosphorylation by mutating Thr to another residue. Which one of these residues will you […]
Induced Pluripotent Stem Cells: How Transcription Factors Induce Pluripotency in Somatic Cells
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- April 13, 2025
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7. In induced pluripotent stem cells: (a) Germ cells are transformed and passaged continuously in culture to maintain a state conducive to future pluripotent cell formation, (b) Oncogenes are added […]
How NH4+ Affects Lysosomal Tracking in Cells: Understanding Protein Degradation and Fluorescence Emission
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- April 13, 2025
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6. A protein cargo X is destined for lysosomal degradation in cells under specific conditions. This can be tracked by red fluorescence emitted by the tag when it reaches lysosomes. […]
How Do Viruses Evade the Human Immune System? Understanding Immune Evasion Mechanisms
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- April 13, 2025
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5. Which one of the following strategies do viruses employ to evade the human immune system? (a) Virus binds to TCR and blocks activation of T-cells, (b) Virus infected cells […]
Understanding Tumor Suppressor Genes and Their Role in Cancer Development
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- April 13, 2025
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4. You have recently observed a mutation in gene X in patients with lung cancer. You have sequenced the gene X in these patients and have observed that (i) both […]
Immunoglobulin IgE and Its Role in Type-I Hypersensitivity Reactions
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- April 13, 2025
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3. Which one of the Immunoglobulins (Ig) cause type-I hypersensitive immune reaction? (a) .IgA, (b) .IgE, (c) .IgG, (d) .IgM, Article: Introduction Type-I hypersensitivity reactions, commonly known as allergic reactions, […]
Understanding the ELISPOT Assay: Measuring Cytokine Release at the Single-Cell Level
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- April 13, 2025
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2. Enzyme-linked immunospot (ELISPOT) assay measures: (a) Cytokine concentration in culture supernatant , (b) Expression of cytokine gene (c) Intracellular cytokine concentration (d) Number of cytokine releasing cells at single […]
Why Topoisomerase is Not Needed in PCR Reactions: An In-depth Explanation
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- April 13, 2025
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1. DNA replication requires DNA-Topoisomerase to remove the supercoiling of DNA that accumulates at the end of a growing replication fork. You wish to perform a PCR amplification of a […]
Understanding Type-I Hypersensitivity: The Role of IgE in Allergic Reactions
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- April 13, 2025
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53. Which one of the Immunoglobulins (Ig) cause type-I hypersensitive immune reaction? (a)IgA (b)IgE (c)IgG (d)IgM Article: Introduction Type-I hypersensitivity refers to allergic reactions that occur rapidly after exposure to […]


