Tag: dbt jrf pyq 2021

30. Gel filtration chromatography separates proteins on the basis of: (a) Affinity tag, (b) Charge, (c) Hydrodynamic volume, (d) Hydrophobicity Introduction Gel filtration chromatography, also known as size exclusion chromatography […]

29. The hypochromic effect is used to estimate the melting temperature for double- stranded DNA. It arises because: (a) Double stranded DNA absorbs more UV light than single stranded DNA, […]

28. In genomics, a contig means: (a) A set of fragments generated through digestion with restriction enzymes, (b) A set of molecular markers used in genetic mapping, (c) A set […]

27. The enzyme used to prevent unwanted self-ligation of DNA molecules during cloning experiments is: (a) Alkaline phosphatase, (b) Reverse transcriptase, (c) Terminal peroxidase, (d) Terminal phosphatase Introduction In DNA […]

26. The presence of Cardiolipin is a characteristic of the membrane of: (a) Endoplasmic reticulum, (b) Lysosomes, (c) Mitochondria, (d) Myelin sheets Introduction Cardiolipin is a unique phospholipid that plays […]

25. During eukaryotic replication, ________ degrades the RNA primer by 5’ – 3’ exonuclease activity. Options:- (a) DNA polymerase V, (b) FEN-1, (c) RNAseH1, (d) Topoisomerase IIB Introduction DNA replication […]

24. A scientist chose Nickel – NTA affinity chromatography to purify a recombinant protein. Which one of the following tag was present in his recombinant protein? (a) Flag, (b) Glutathione-S-transferase, […]

23. A geneticist interested in immune function induces random mutations in a number ofspecific genes in mice and then determines which of the resulting mutant mice have impaired immune function. […]

22. Genes that are related through gene duplication events are: (a) Analogs, (b) Homologs, (c) Orthologs, (d) Paralogs Introduction Gene duplication is a fundamental mechanism in molecular evolution. It plays […]

21. Which one of the following is used to validate the secondary structure of proteins? (a) Dot plot, (b) Neural network, (c) Ramachandran plot, (d) Sigma plot Introduction Proteins are […]