Tag: DBT JRF PYQ 2020

A class of temperature sensitive E. coli mutants defective in DNA replication were identified that ceased replication immediately upon increase in temperature. Which of the following processes are likely to […]

Accuracy of Protein Structure Prediction can be assessed using tools like 1.WHATIF 2. COOT 3. BLAST 4. PHENIX Detailed Explanation:Correct Answer:2. variation at a single nucleotide position observed in at […]

What is Single Nucleotide Polymorphism (SNP)? 1.variation at a single nucleotide position observed in 100% population 2. variation at a single nucleotide position observed in at least 1% population 3. […]

Nucleosides isolated from a Caribbean sponge, Cryptotethyacrypta, were the basis for the synthesis of the antiviral 1. Avarol 2. Amantadine 3. Abacavir 4. Acyclovir Detailed Explanation: Correct Answer: 4. Acyclovir […]

Immobilization of enzymes to water insoluble, anionic porous carriers often results in an apparent shift in the pH optima of the enzyme. The physico-chemical interaction likely to cause such a […]

Which autoimmune disease is caused by production of autoantibodies and autoreactive T cellsagainst DNA and chromatin proteins? 1. Systemic lupus erythematosus 2. Graves’ Disease 3. Sjögren syndrome 4. Multiple Sclerosis […]

A CDNA encoding a human protein of interest was cloned in a bacterial expression vector and introduced into bacterial cells for expression. However, no expression of the human protein of […]

A student added a 5′ exonuclease enzyme instead of a restriction enzyme to digest his purifiedplasmid DNA sample. What is he likely to observe when he runs his plasmid digest […]

Which of the following is NOT a feature of bacterial DNA replication? 1. semi-conservative 2. semi-discontinuous 3. chain growth in the 5′ -> 3′ direction 4. Unidirectional Detailed Explanation: Correct […]

Sequence-specific recognition of DNA by proteins occurs primarily through the 1. major groove 2. minor groove 3. Histones 4. polyphosphate backbone Detailed Explanation: Correct Answer: 1. Major groove What is […]