In the biosynthesis of purine: (1) All N atoms, C4 and C5 from Aspartic acid (2) N1 is from aspartic acid; N3 and N9 are from Glutamine side- chain; N7, […]
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Amino Acid Labeling in Purine Biosynthesis: Which Amino Acid Does NOT Incorporate 15N into Purines?
A cell line deficient in salvage pathway for nucleotide biosynthesis was fed with medium containing 15N labelled amino acids. Purines were then extracted. Treatment with which one of the following […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Matching Tryptophan Biosynthesis Mutants with Anthranilate Fluorescence Phenotypes in Plants
The first few steps of the tryptophan biosynthetic pathway in plants along with the available mutants is provided below Column X represents genotypes and Column Y summarizes the anthranilate fluorescence […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Isotope Incorporation Pattern in Valine Biosynthesis from Mixed Pyruvate Substrates
The first step in the biosynthesis of valine begines with enzyme catalyzed condensation of two molecules of pyruvic acid. If an equimolar mixture of 13CH3COCOOH and 12CH3COCOOH are used as […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Alkaptonuria: Enzyme Deficiency Causing the Inborn Error of Amino Acid Metabolism
The inborn error of amino acid metabolism, alkaptonuria. is due to the lack of one of the following enzymes: (1) Fumaryl acetoacetate hydrolase (2) α-keto acid decarboxylase (3) Hornogentisate oxidase […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Key Facts About Biosynthesis: Clarifying Common Misconceptions on Palmitate and Amino Acid Formation
Choose the correct answer from the following statements on biosynthesis. (1) In the biosynthesis of palmitate, all the carbon atoms are derived from activated malonate. (2) The amino acids Met, […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Biosynthesis of Tyrosine: Identifying Key Intermediates and Cofactors in the Shikimate Pathway
Biosynthesis of tyrosine is detailed below: Shikimic acid – A → shikimic acid-5-phosphate -B → C → chorismic acid → prephenic acid → D → transaminase → tyrosine. Identify A, […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Melittin Activation of Phospholipase in Bee Venom: Targeting Phospholipase A2 to Generate Arachidonic Acid
Sting of a bee causes pain, redness and swelling. Melittin is a major peptide in bee venom. Melittin is a membrane binding peptide that is involved in activating phospholipases in […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Major Regulatory Step in Cholesterol Biosynthesis: The Role of HMG-CoA Reductase
Major regulatory step in cholesterol biosynthesis is (1) HMG coA reductase (2) HMG CoA synthase (3) Thiokinase (4) Mevalonate kinase Cholesterol is an essential lipid molecule involved in maintaining cell […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Number of β-Oxidation Cycles Required to Completely Hydrolyze Arachidonic Acid into Acetyl CoA
Number of cycles required to completely hydrolyze Arachidonic acid into acetyl CoA is (1) 9 (2) 8 (3) 10 (4) 11 Arachidonic acid is a polyunsaturated fatty acid with 20 […]
