Inbreeding for 5 generations led to production of homozygous transgenic mice. However, these homozygous males or females were infertile. Which of the following approach is most preferable and economical to […]
Tag: CSIR NET Life Science Previous Year Questions on applied biology
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CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Why DMEM turns yellow in liver cell culture
A student added DMEM culture medium which was pink in colour to growing liver cells. Three days later the medium colour was yellow. This indicated (1) change in cell morphology […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Method to detect mycoplasma contamination in mammalian cell cultures
Which is best method for checking mycoplasma contamination in a mammalian cell line? (1) Southern Hybridization (2) ELISA (3) PCR (4) Western Hybridization The best method is (3) PCR. Why PCR […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Reagents used to dissociate primary tissues into single cells
To prepare individual tissue cells from a primary culture, the cell-cell and cell-matrix interaction must be broken. To achieve this would NOT use: (1) EDTA […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Key facts about maintaining mammalian cells in vitro
Which one of the following statements is NOT correct for propagation and maintenance of mammalian cells in vitro? (1) Transformed cell lines do need external supply of serum to grow. […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Procedure to develop a transgenic mouse by pronuclear microinjection
The following statements are made regarding developing a transgenic mouse: A. The transgenic mouse thus born will be a homozygous transgenic animal and can be maintained by crossing with another […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Best system and starting material to purify secreted human growth hormone
14. Animal cell cultures are frequently used for production of therapeutic proteins. NIH3T3 (a fibroblast cell line) and CHO (Chinese hamster ovarian cell line) are some of the popular cell […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Standard method for producing transgenic animals
13. Which one of the following techniques is generally used to produce transgenic animals? (1) Processed mRNA containing only exons are introduced into blastocyst stage embryo. (2) Entire foreign nucleus […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Factor required to maintain embryonic stem cells in a totipotent/pluripotent state
To keep them in a totipotent state, embryonic stem cells need to be maintained in a medium supplemented with (1) growth hormone (2) leukemia inhibiting factor (3) nestin (4) insulin […]
CSIR NET Life Science Previous Year Questions and Solution on Animal Cell Culture and Transgenic Animals
Antibiotic selection of embryonic stem cells in transgenic mouse production
For developing transgenic mice, embryonic stem cells are engineered to express the transgene. These cells are selected by (1) novobiocin. (2) neomycin. (3) tetracycline. (4) penicillin. The correct answer is (2) […]
