A student noted the following points regarding Agrobacterium tumefaciens: A. A. tumefaciens is a gram-negative soil bacterium. B. Opine catabolism genes are present in T- DNA region of Ti-plasmid. C. […]
Tag: CSIR NET Life Science Previous Year Questions on applied biology
Tag: CSIR NET Life Science Previous Year Questions on applied biology
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Opines and Agrobacterium virulence
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- November 28, 2025
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Which one of the following statements is correct? (1) None of the virulence genes of Agrobacterium tumefaciens are expressed constitutively. (2) Integration of T-DNA with the nuclear genome of plant […]
Essential and non‑essential genes for Agrobacterium‑mediated plant transformation
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- November 28, 2025
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Agrobacterium tumefaciens is frequently used as a vector to create transgenic plants. Under laboratory conditions Agrobacterium – mediated plant transformation does not require (1) host plant genes (2) bacterial type […]
Interpreting T‑DNA hormone genes in Agrobacterium tumefaciens
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- November 28, 2025
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The T-DNA region of the Ti plasmid of Agrobacterium tumefaciens harbors two genes: X and Y. Mutation of gene ‘X’ produces a rooty tumour while mutation of gene ‘Y’ produces […]
Which molecule activates Agrobacterium vir genes?
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- November 28, 2025
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A set of virulence genes (vir genes), located in the Agrobacterium Ti-plasmid, is activated by (1) octopine (2) nopaline […]
Why acetosyringone is used in Agrobacterium‑mediated plant transformation
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- November 28, 2025
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Treatment of acetosyringone is given during transfer of transgene using Agrobacterium as vector. The rationale behind this is that acetosyringone (1) Helps in anchorage of bacteria to plant cell wall […]
Bacillus thuringiensis as a biocontrol agent against insect pests
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- November 28, 2025
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Which microbe has been used against insect plant pathogens? (1) Agrobacterium tumefaciens (2) Agrobacterium rhizogenes (3) Bacillus thuringenesis (4) Fusarium nudum Why Bacillus thuringiensis is correct Bacillus thuringiensis (Bt) is […]
Agrobacterium tumefaciens as the primary tool for generating transgenic plants
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- November 28, 2025
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Which of the following organism have been extensively used in generation of transgenic plants? (1) Agrobacterium tumfaciens (2) Bacilus thuringensis (3) Baculo viruses […]
Which hormone delays Arabidopsis seed germination on MS medium?
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- November 28, 2025
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Arabidopsis thaliana seeds were planted on Murashige Skoog (MS) plates with or without a hormone added to the medium. Seeds were found to germinate late in the hormone containing MS […]
Key facts about protoplast fusion in plant tissue culture
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- November 28, 2025
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Protoplast fusion is used in plant tissue culture for various applications. In protoplast fusion: A. naked plant cells are used. B. transfer of organelles is not possible. C. partial genome […]


