A transposon carrying a promoterless ß-galactosidase (lac Z) was used to create insertional mutation in the vir region of Ti-plasmid of Agrobacterium tumefaciens. All the mutants in which lac Z […]
Tag: CSIR NET Life Science Previous Year Questions on applied biology
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CSIR NET Life Science Previous Year Questions and Solution on applied biology
Identifying the incorrect step in Agrobacterium infection of plant cells
Which of the following statement is NOT true during infection of plant cell by Agrobacterium? (1) The protein products of virulence genes Vir A and VirG perceives acetosyringone (2) The […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Which gene should be silenced to increase tomato shelf‑life?
To increase the shelf-life of tomato, which of the following genes should be silenced? (1) ACC synthase (2) EPSP synthase (3) […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Which Agrobacterium Vir proteins act inside plant cells?
Agrobacterium Ti plasmid vectors are used to generate transgenic plants. The following are examples of vir gene encoded proteins that are important for transfer of T-DNA in to plants. A- […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Functions of Vir proteins during Agrobacterium T-DNA transfer
Match the following Vir proteins with their correct function during Agrobacterium mediated transfer of T- DNA to plant cells. (1) A-(i), B-(ii), C-(iii), D-(iv) (2) A-(iv). B-(i). C-(ii), D-(iii) (3) […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Sequence of events in Agrobacterium T-DNA transfer
Given below are four statements related to Agrobacterium-mediated transfer of T-DNA into plant cells: A. Production of single-stranded T-DNA by VirD1 and VirD2 proteins. B. Interaction of VirE2 with VIP1 […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Why Agrobacterium-based transgenic plants do not form tumors
Agrobacterium tumefaciens, also known as natural genetic engineers, causes crown-gall disease in plants. However, when the same bacteria are used to raise transgenic plants with improved agronomic traits, no such […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Key genetic elements in Agrobacterium-mediated plant transformation
Following are some statements related to raising transgenic plants A. T-DNA of Agrobacterium contains oncogenes and opine synthesis genes B. All 8 operons present in vir region of Agrobacterium is […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Key facts for designing Agrobacterium-mediated plant transformation experiments
While designing an experiment for Agrobacterium mediated plant transformation, a student noted down the following points: A. Ti and Ri plasmids induce crown gall and hairy root disease, respectively B. […]
CSIR NET Life Science Previous Year Questions and Solution on applied biology
Understanding Ti plasmid regions and Agrobacterium diseases in plant transformation
Following are certain statements regarding the use of Agrobacterium in plant transformation. A. A. tumefaciens causes crown gall disease and A. rhizogenes causes hairy root disease. B. Region A in […]
