Category: GATE Biotechnology Previous Year Papers with Solutions

Q.50 E. coli cultivated at 298 K uptakes an uncharged compound (A) by passive diffusion. The intracellular and extracellular concentrations of A are 0.001 M and 0.1 M, respectively. If […]

Q.49 Aqueous two–phase extraction is used to recover α–amylase from a solution. Apolypropylene glycol–dextran mixture is added and the solution separates intoupper and lower phases. The partition coefficient is 4.0 […]

Q.48 E. coli is cultivated in a chemostat operated at a dilution rate of 0.2 h−1. The values of biomass yield due to oxygen consumption and the steady state biomass […]

Q.47 Temperature of a reaction with an activation energy value of 15 kcal.mol−1 is increased from 300 K to 310 K. If the value of the ideal gas constant (R) […]

Q.46 The following reaction represents biomass synthesis from hexadecane C16H34 + 12.5O2 + 2.13NH3 → 10.6CH1.66O0.27N0.27 + 5.37CO2 + 11.4H2O where CH1.66O0.27N0.27 represents the biomass. The value of respiratory quotient […]

Q.45 Which of the following vector(s) is(are) used to clone a DNA fragment of size220 kb?(A) Bacterial artificial chromosome(B) Yeast artificial chromosome(C) Cosmids(D) pUC19 plasmid Cloning vectors like BAC and […]

Q.44 Direct DNA transfer method(s) used for plant genetic engineering is(are)(A) microparticle bombardment(B) electroporation(C) polyethylene glycol treatment(D) Agrobacterium–mediated transformation Direct DNA transfer methods for plant genetic engineering include microparticle bombardment, […]

Q.43 Which of the following is(are) plant protoplast fusogenic agent(s)?(A) Sodium nitrate(B) Polyvinyl alcohol(C) Polyethylene glycol(D) Bromoxynil Polyethylene glycol (C) is the primary plant protoplast fusogenic agent among the options, […]

Q.42 Which of the following statement(s) is(are) TRUE about fluoroquinolone drugs?(A) They contain quinolone ring(s)(B) They inhibit RNA polymerase(C) They bind to bacterial topoisomerase(D) They bind to 23S rRNA within […]

Q.41 Which of the following statement(s) is(are) TRUE about induced pluripotent stemcells?(A) They can self–renew(B) They require specific signals to maintain their stemness(C) They cannot be genetically manipulated(D) They can […]