Category: CSIR NET Life Science Previous Year Questions and Solution on applied biology

Inbreeding for 5 generations led to production of homozygous transgenic mice. However, these homozygous males or females were infertile. Which of the following approach is most preferable and economical to […]

A student added DMEM culture medium which was pink in colour to growing liver cells. Three days later the medium colour was yellow. This indicated (1) change in cell morphology […]

Which is best method for checking mycoplasma contamination in a mammalian cell line? (1) Southern Hybridization (2) ELISA (3) PCR (4) Western Hybridization The best method is (3) PCR. Why PCR […]

To prepare individual tissue cells from a primary culture, the cell-cell and cell-matrix interaction must be broken. To achieve this would NOT use: (1) EDTA            […]

Which one of the following statements is NOT correct for propagation and maintenance of mammalian cells in vitro? (1) Transformed cell lines do need external supply of serum to grow. […]

The following statements are made regarding developing a transgenic mouse: A. The transgenic mouse thus born will be a homozygous transgenic animal and can be maintained by crossing with another […]

14. Animal cell cultures are frequently used for production of therapeutic proteins. NIH3T3 (a fibroblast cell line) and CHO (Chinese hamster ovarian cell line) are some of the popular cell […]

13. Which one of the following techniques is generally used to produce transgenic animals? (1) Processed mRNA containing only exons are introduced into blastocyst stage embryo. (2) Entire foreign nucleus […]

To keep them in a totipotent state, embryonic stem cells need to be maintained in a medium supplemented with (1) growth hormone (2) leukemia inhibiting factor (3) nestin (4) insulin […]

For developing transgenic mice, embryonic stem cells are engineered to express the transgene. These cells are selected by (1) novobiocin. (2) neomycin. (3) tetracycline. (4) penicillin. The correct answer is (2) […]