In an electrophoresis system, using a native gel (agarose) proteins are separated due to difference in total charge. What decides the charge of protein ? (a) Voltage (b) Buffer’s pH […]
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CSIR NET Previous Year Questions Gel Electrophoresis
2D gel electrophoresis Isoelectric Focusing (IEF) and SDS-PAGE
Which of the following statement about 2D gel electrophoresis is correct? (a) SDS gel electrophoresis and isoelectric focusing both separate native proteins (b) SDS gel electrophoresis and isoelectric focusing both […]
CSIR NET Previous Year Questions Gel Electrophoresis
Isoelectric Focusing-The separation of proteins is only based on charge
Which of the following statement about isoelectric focusing is correct (a) Proteins separated by isoelectric focusing cannot be tested for biological activity (b) Proteins separated by it can be tested […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS PAGE separate proteins based on their molecular weight
Which of the following statements about SDS PAGE is correct ? a) Wanted proteins can be tested for their biological activity after separation by SDS PAGE (b) Proteins are solubilised […]
CSIR NET Previous Year Questions Gel Electrophoresis
Free flow electrophoresis-separated according to their electrophoretic mobility
If proteins are separated according to their electrophoretic mobility then type of electrophoresis is (a) SDS PAGE (b) Free flow electrophoresis (c) Electro focusing (d) Affinity electrophoresis ✅ Correct […]
CSIR NET Previous Year Questions Gel Electrophoresis
SDS-PAGE -Sodium Dodecyl Sulfate – Polyacrylamide Gel Electrophoresis
In SDS PAGE the protein sample is first (a) Treated with reducing agent and then with anionic detergent followed by fractionation by electrophoresis (b) Fractionated by electrophoresis then treated with […]
CSIR NET Previous Year Questions Gel Electrophoresis
The electrophoresis technique that use isoelectric focusing is
The electrophoresis technique that use isoelectric focusing is (a) AGE (b) PFGE (c) 2D PAGE (d) SDS PAGE Correct answer: (C) 2D PAGE Explanation: Isoelectric focusing (IEF) is a technique […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two dimensional gel electrophoresis is a technique for separating proteins
Two dimensional gel electrophoresis is a technique for separating proteins (A) Based on charge (B) Based on mass (C) Based on both charge and mass (D) Based on its pI […]
CSIR NET Previous Year Questions Gel Electrophoresis
APS (Ammonium persulphate) is used in SDS-PAGE
APS (Ammonium persulphate) is used in SDS-PAGE for (A) preventing oxidation (B) cross-linking (C) its role as a catalyst (D) free radical formation Correct answer: (D) Free radical formation Explanation: […]
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Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands
Electrophoresis of a purified protein in SDS-PAGE in the presence of b-marcaptoethanol yields two bands of 35 kDa and 45 kDa. However, in a gel filtration chromatography, the same protein […]
