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47. Irrespective of the chromosomal configuration, a single X chromosome remains active in all diploid human somatic cell lines. Which one of the following mechanisms best accounts for the above […]

46. In an experiment, the student has infected mammalian host cell with cytoplasmic RNA virus. The virus growth was monitored by measuring the intracellular viral RNA at different time intervals. […]

45. mRNA of a gene was depleted in human cells using siRNA that arrest cells in the G2 phase of the cell cycle. In order to test whether the G2 […]

44. Virus induced gene silencing (VIGS) is a process that takes advantage of the RNAi mediated antiviral defense mechanism. Which one of the following ultimately guides siRNA to degrade the […]

43. siRNAs and miRNAs are used for achieving gene silencing. Although, major steps are similar there are distinct differences in the key players of the two processing pathways. Following statements […]

42. MicroRNAs (miRNAs) have recently been shown to play significant role in the fine tuning of gene expression. Some miRNAs induce gene silencing by binding to mRNAs and inducing inhibition […]

41. RNA interference is mediated by both siRNA and miRNA. Which one of the following statement about them is NOT true? (1) Both siRNA and miRNA are processed by DICER. […]

40. Which of the following statement is NOT true about small interfering RNA (siRNA)? (1) siRNA has 21-25 nucleotide sequence with 2 nucleotide overhang at 3′ end (2) siRNA is […]

39. In an experiment, intact chromatin was isolated and digested with micrococcal nuclease in independent tubes for 30 min, 1 h, 2h, and 4h. Further, the DNA was purified from […]

38 human, protein coding genes are mainly organized “exons” and “introns’’. There are intergenic regions that transcribe into various types of non-coding RNA (nottranslating into protein). Some introns may harbor […]