20. A practical class was going on where the students were demonstrating ATP synthesis in vitro using active mitochondria. Some students added one of the following to their tubes A. […]
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Effects of Rotenone on Mitochondrial Electron Transport Chain: ATP Blockade and ROS Generation
19. Rotenone is an inhibitor of the electron transport chain. The addition of rotenone to cells results in which of the following? (1) Generation of mitochondrial reactive oxygen species and […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Oxygen Consumption Profiles in Mitochondrial Respiration Experiments
18. In a mitochondrial respiration experiment, a researcher observed the following profile of oxygen consumption upon addition of following compounds at times I, II and III. (a) ADP + Pi […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Why Submitochondrial Particles Facilitate Study of Electron Transport and ATP Synthesis
17. The respiratory chain is relatively inaccessible to experimental manipulation in intact mitochondria. Upon disrupting mitochondria with ultrasound, however, it is possible to isolate functional sub mitochondrial particles, which consist […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How Coupling of Reaction Centers Is Achieved in Oxidative Phosphorylation: The Role of Proton Pumping
16. Coupling of the reaction centers of oxidative phosphorylation is achieved by which one of the following? (1) Making a complex of all four reaction centers. (2) Locating all four […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Identifying Incorrect Statements on Biosynthesis, Reaction Spontaneity, and Proton Motive Force
15. From the following statements: A. Biosynthesis of proteins and nucleic acids from precursors results in production of chemical energy in the form of ATP. NAOH. NADPH and FADH2 B. […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Chemical Inhibitors of the Mitochondrial Electron Transport Chain: Role of Azides
14. Among the following which chemical inhibits the mitochondrial electron transport chain- (1) Streptomycin (2) Nystatin (3) Azides (4) Penicillin The mitochondrial electron transport chain (ETC) is essential for […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Which Protein Acts as an Energy Transducer? Exploring the Role of Bacteriorhodopsin
13. Which of the following proteins acts as an energy transducer? (1) G-protein. (2) Bacteriorhodopsin. (3) Hemoglobin. (4) Heat shock protein. Energy transducers are proteins that convert one form […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Electron Transfer from NADH and FADH2 to Oxygen: The Role of Mitochondrial Inner Membrane
12. Electron transfer from donors such as NADH and FADH2 to O2 occurs in (1) membranes of ER, chloroplast and mitochondria (2) chloroplast only (3) mitochondria only (4) organellar membranes […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How Proton Motive Force Is Generated in Mitochondria During Oxidative Phosphorylation
11. Proton motive force during oxidative phosphorylation is generated in mitochondria by (1) exchanging protons for sodium ions (2) pumping protons out into intermembrane space (3) pumping hydroxyl ions into […]
