7. Among the following in which mutant gene of l phages will form clear bacterial plaques (1) cl– (2) cro- (3) INT– (4) XIS– Explore how mutations in the lambda […]
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Termination of Bacterial Chromosomal DNA Replication Occurs at Several Specific Loci
3. In bacteria chromosomal DNA replication stops at (1) one specific locus. (2) several specific loci. (3) a single locus, randomly. (4) from several loci, randomly. Introduction In bacteria, […]
How UV Radiation Induces Lambda Prophage from Lysogenic E. coli Cultures
6. A culture of an E. coli strain that is lysogenic for phage lambda is grown at 320C Induction of the prophage from the host chromosome will occur when the […]
Why Origins of Replication Are Typically AT-Rich Sequences: Understanding DNA Replication Initiation
Origin of replication usually contains (1) GC rich sequences. (2) both AT and GC rich sequences. (3) no particular stretch of sequences. (4) AT rich sequences. Introduction The origin […]
Substrate for DNA Synthesis: Why Deoxynucleoside Triphosphates (dNTPs) Are Essential
Substrate for DNA synthesis is? (1) Deoxy Nucleotide tri phosphate (2) Deoxy Nucleoside tri phosphate (3) Nucleoside pyrophosphate (4) Ribonucleotide tri phosphate Introduction DNA synthesis is a fundamental biological […]
How Bacteriophage T4 Hijacks Host RNA Polymerase for Viral Gene Transcription in E. coli
5. T4 bacteriophage after infecting E. coli generally hijack host machinery for transcription of its own genes. It is done by (1) Degrading host RNA polymerase (2) Modifying host RNA […]
How Bacteriophage T4 Transcribes Viral Genes Immediately After Infection
4. Bacteriophage T4 transcribe the viral genes immediately after infection using (1) host RNA polymerase. (2) viral RNA polymerase. (3) modified host RNA polymerase. (4) RNA transcriptase. Discover how bacteriophage […]
Autoradiography Patterns In Vitro Translation with Cyanogen Bromide Cleavage
11. An in vitro translation system capable of incorporating ~8 amino acids was programmed to translate a single mRNA that codes for an alanine-rich (35% alanine with uniform distribution of […]
Chromatin Immunoprecipitation (ChIP) Assay
10. A reporter cell line with stably integrated retroviral promoter-luciferase construct was transfected with an expression vector for a cellular protein. The protein seems to regulate the activation of retroviral […]
How to Identify Enhancer Sequences Using Reporter Constructs
9. A researcher wanted to identify the enhancer sequences of a newly discovered gene. Shown below are the relevant regions of some of the reporter constructs the researcher designed to […]


