The following represents sequences of different alleles of a gene found in family represented by mother, (allele1/ allele2), father (allele1/allele 2) and their two sons: Son1 (allele1/allele 2) and Son […]
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Microsatellite DNA Fingerprinting
73. Microsatellite are used as marker for identifying individuals via DNA fingerprinting os the alleles may differ in the number of repeals. From the Southern blot shown below identify the […]
Late-onset autosomal dominant pedigree analysis with RFLP
72. The pedigree given below follows the inheritance pattern of a late-onset (after age of 30 years) genetic disease that is 100% penetrant. Affected individuals are indicated by a solid […]
Microsatellite Marker Linkage Analysis
71. An analysis of four microsatellite markers was carried out in a family showing a genetic disorder. The results are summarized below Based on the above, which ot the markers […]
Sickle Cell Anemia Restriction Digest Problem
70. Sickle cell anemia is a recessive genetic disease caused due to a point mutation in the 6th codon abolishing one of the MspIIendonuclease digestion site present in the ß-globin […]
RFLP Analysis of β‑Globin MstII Sites
69. In normal individuals, there are three Mstll restrictionsites. two flanking the ß-globin gene and one within thegene. In individuals affected by a disease, a singlenucleotide polymorphism in the ß-globin […]
DNA Marker Gel
68. The following picture represents a gel profile of a pair of DNA markers observed P1 and P2, their F1 progeny and F2 progeny. Four different profiles were observed in […]
AFLP codominant markers in doubled haploid progeny
67. The AFLP technique generates polymorphic DNA fragments that are generally scored as dominant markers. However, a pair of DNA fragments (say ‘a’and ‘b’) generate by AFLP can be termed […]
AFLP bands, doubled haploids, and allelism
66. The following represents selected AFLP bands (l to V) observed in parents (P1 and P2), Fl progeny and 20 doubled haploid (DH) progeny developed from the F1. DH are […]
Doubled Haploids, Marker–Trait Linkage and Recombination
64. Doubled haploids (DH) are plants derived from single immature pollen and doubled artificially to form diploids. A DH population was created from progeny derived from a cross between two […]


