124. Recombinant Interferon Gamma is commercially produced by cell culture of
1. T3 fibroblast cells
2. Chinese hamster ovary cells
3. Peripheral lymphocyte cells
4. Human leucocyte cells

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Correct Answer:

2. Chinese hamster ovary cells

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Explanation:

Recombinant Interferon Gamma is a cytokine that plays a key role in immune responses, and it is commercially produced using cell culture techniques. To produce recombinant proteins like Interferon Gamma, Chinese hamster ovary (CHO) cells are commonly used. These cells are a preferred choice for producing large quantities of recombinant proteins due to their ability to correctly fold complex proteins and perform post-translational modifications such as glycosylation.

Here’s a closer look at why CHO cells are used:

1. CHO Cells: Chinese hamster ovary cells are widely used in the biotechnology industry for the production of therapeutic proteins, including recombinant Interferon Gamma. CHO cells are ideal for protein expression due to their high growth rates, ability to grow in suspension culture, and their capacity to produce human-like glycosylated proteins.

2. Other Options:

   • T3 fibroblast cells: These cells are not commonly used for recombinant protein production. While they are used in some specific contexts (like in early experiments), they are not standard for large-scale recombinant protein production.

   • Peripheral lymphocyte cells: Lymphocytes, while important for immune response, are not typically used for large-scale recombinant protein production due to limitations in culture and production efficiency.

   • Human leucocyte cells: These are blood cells involved in immune function and are not used for recombinant protein production due to challenges in culturing and maintaining them for large-scale production.

Thus, CHO cells remain the gold standard for producing recombinant Interferon Gamma due to their efficiency and ability to produce complex, biologically active proteins.

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Conclusion:

The correct answer is Chinese hamster ovary cells (CHO cells), which are used for the large-scale production of recombinant Interferon Gamma in cell cultures. These cells are preferred in biotechnology due to their suitability for producing complex proteins with human-like modifications.