Q83. Match the microorganism in Group I with the application in Group II Group I Group II P. Aspergillus oryzae Q. Brevibacterium flavum R. Thiobacillus ferrooxidans S. Saccharomyces cerevisiae T. Rhizobium meliloti 1. Metal ore leaching 2. Glucoamylase producer 3. Bread making 4. Glutamic acid producer 5. Penicillin producer 6. Symbiotic nitrogen fixer P-1, Q-6, R-4, S-5, T-2 P-2, Q-4, R-1, S-3, T-6 P-4, Q-1, R-6, S-3, T-5 P-6, Q-2, R-3, S-5, T-1

Q83. Match the microorganism in Group I with the application in Group II

Group I Group II
P. Aspergillus oryzae
Q. Brevibacterium flavum
R. Thiobacillus ferrooxidans
S. Saccharomyces cerevisiae
T. Rhizobium meliloti
1. Metal ore leaching
2. Glucoamylase producer
3. Bread making
4. Glutamic acid producer
5. Penicillin producer
6. Symbiotic nitrogen fixer
  1. P-1, Q-6, R-4, S-5, T-2
  2. P-2, Q-4, R-1, S-3, T-6
  3. P-4, Q-1, R-6, S-3, T-5
  4. P-6, Q-2, R-3, S-5, T-1

    Microorganisms Matching: Aspergillus oryzae to Rhizobium meliloti Applications

    The correct answer for this matching question is P-2, Q-4, R-1, S-3, T-6. This pairs each microbe from Group I with its primary industrial or biological role in Group II.

    Correct Matches

    • P. Aspergillus oryzae → 2. Glucoamylase producer: This fungus secretes glucoamylase (amyloglucosidase), an enzyme that hydrolyzes starch to glucose, vital for saccharification in biofuel and food industries like sake production.

    • Q. Brevibacterium flavum → 4. Glutamic acid producer: Used in submerged fermentation for L-glutamic acid (MSG precursor), a key amino acid in food flavoring via Corynebacterium-like strains.

    • R. Thiobacillus ferrooxidans → 1. Metal ore leaching: This acidophilic bacterium oxidizes Fe²⁺ to Fe³⁺ and sulfur to sulfuric acid, bioleaching copper, gold, and uranium ores in biomining.

    • S. Saccharomyces cerevisiae → 3. Bread making: The baker’s yeast ferments dough sugars to CO₂ and ethanol, causing bread to rise through leavening.

    • T. Rhizobium meliloti → 6. Symbiotic nitrogen fixer: Forms nodules on alfalfa (Medicago sativa), fixing atmospheric N₂ into ammonia via nitrogenase for plant growth.

    Option Analysis

    Option 1: P-1, Q-6, R-4, S-5, T-2
    Incorrect. Aspergillus oryzae does not leach metals (that’s Thiobacillus); Brevibacterium isn’t a nitrogen fixer; Saccharomyces doesn’t produce penicillin (Penicillium does); Rhizobium doesn’t make glucoamylase.

    Option 2: P-2, Q-4, R-1, S-3, T-6
    Correct. All matches align with established microbial biotechnology roles: enzyme production, amino acid fermentation, bioleaching, yeast leavening, and symbiosis.

    Option 3: P-4, Q-1, R-6, S-3, T-5
    Incorrect. Aspergillus oryzae doesn’t produce glutamic acid or penicillin; Brevibacterium doesn’t leach ores; Thiobacillus ferrooxidans isn’t a nitrogen fixer; Rhizobium meliloti doesn’t produce penicillin.

    Option 4: P-6, Q-2, R-3, S-5, T-1
    Incorrect. Aspergillus oryzae isn’t primarily a nitrogen fixer; Brevibacterium flavum doesn’t produce glucoamylase; Thiobacillus doesn’t make bread; Saccharomyces cerevisiae doesn’t produce penicillin; Rhizobium doesn’t leach metals.

    Microorganism Correct Application Common Use Case
    Aspergillus oryzae Glucoamylase producer Starch hydrolysis in fermentation 
    Brevibacterium flavum Glutamic acid producer MSG manufacturing 
    Thiobacillus ferrooxidans Metal ore leaching Biomining copper ores 
    Saccharomyces cerevisiae Bread making Dough leavening 
    Rhizobium meliloti Symbiotic nitrogen fixer Alfalfa nodulation 

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