Q.5 The product commercially produced by animal cell culture is
- (A) insulin
- (B) tissue plasminogen activator
- (C) interferon
- (D) hepatitis B vaccine
The product commercially produced by animal cell culture is tissue plasminogen activator (tPA), a critical thrombolytic agent manufactured using mammalian cell lines like CHO cells. This SEO-optimized article addresses the MCQ: The product commercially produced by animal cell culture is (A) insulin, (B) tissue plasminogen activator, (C) interferon, or (D) hepatitis B vaccine—tailored for biotechnology students focused on cell culture and bioprocess engineering.
Correct Answer: Option (B) Tissue Plasminogen Activator
Tissue plasminogen activator (tPA), such as Alteplase, is commercially produced by animal cell culture, specifically recombinant CHO or HEK cells, enabling proper glycosylation essential for its clot-dissolving activity in stroke and heart attack treatments.
Approved by FDA in 1987, tPA production scales via bioreactors mimicking microbial fermentation, yielding grams per liter—key in biopharmaceuticals where animal cells post-translationally modify proteins beyond bacterial limits.
Explanation of All Options
Each option highlights production systems in biotech; animal cell culture excels for complex eukaryotic proteins.
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(A) Insulin: Incorrect. Recombinant human insulin is produced in bacterial (E. coli) or yeast systems for simplicity and cost, not animal cells.[ from context]
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(B) Tissue plasminogen activator: Correct. Requires mammalian glycosylation; commercial tPA like Activase uses CHO cells for therapeutic efficacy.
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(C) Interferon: Incorrect. Early alfa-interferons used bacteria; some beta versions use CHO, but not the hallmark “commercially produced by animal cell culture.”
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(D) Hepatitis B vaccine: Incorrect. It’s a yeast (Saccharomyces)-produced HBsAg recombinant protein, avoiding animal cell complexities.
Option Production Host Key Reason Correct/Incorrect (A) Insulin Bacteria/Yeast Simple peptide, no glycosylation needed Incorrect (B) Tissue plasminogen activator Animal (CHO/HEK) Complex glycosylation for activity Correct (C) Interferon Mostly Bacteria Cost-effective for cytokines Incorrect (D) Hepatitis B vaccine Yeast Viral surface antigen Incorrect Relevance in Bioengineering
Animal cell culture drives 70% of biopharma, aligning with your enzyme kinetics and mammalian culture expertise—vital for scaling in Jaipur’s growing biotech sector.
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