Which of these is used as a tracking dye in SDS PAGE of protein
(a) Bromo phenol blue
(b) Xylene cyanol
(c) Orange G
(d) All of the above
The correct answer is:
(d) All of the above
Explanation:
In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), tracking dyes are used to monitor the progress of electrophoresis and ensure proper migration of proteins. Different dyes migrate at different speeds, allowing flexibility based on experimental needs.
- Bromophenol Blue (BPB) → Most commonly used tracking dye. It migrates faster than most proteins.
- Xylene Cyanol → Moves slower than bromophenol blue and is useful for tracking larger proteins.
- Orange G → Moves faster than bromophenol blue and is sometimes used when tracking smaller peptides or when alternative dyes are needed.
Since all three dyes can be used as tracking dyes in SDS-PAGE, the correct answer is:
(d) All of the above ✅



76 Comments
Suman bhakar
February 26, 2025Done
Mahima Sharma
August 24, 2025Bpb, xylene cyanol, orang g
Arushi
February 26, 2025☑️👍
Parul
February 27, 2025Done sir
Pooja jat
February 27, 2025Easily understood the question…,,, done!
Lavanya Sharma
February 27, 2025Got it sir!
Lavanya Sharma
February 27, 2025✅👍🏻
Prami Masih
February 27, 2025Awesome sir
Akshay mahawar
February 27, 2025Done 👍
yogesh sharma
February 27, 2025Easily explained sir
Lokesh kumawat
March 1, 2025Done
Abhilasha
March 1, 2025Done
Nisha
March 1, 2025Done
VIKRAM GAHLOT
March 7, 2025👍
Anita choudhary
April 19, 2025Done👍
Anurag Giri
August 13, 2025Ans 4 all are correct
Aman Choudhary
August 13, 2025BPB, Xylene Cyanol and orange G is used
Khushi Agarwal
August 14, 2025All of above
Dharmpal Swami
August 14, 2025All of these are use for SDS page
Neelam Sharma
August 14, 2025All of these
Anjani sharma
August 14, 2025All of these are used as tracking dye
Karishma Don
August 14, 2025all the above dyes, as they are anionic
Mitali
August 14, 2025all three dyes can be used as tracking dyes in SDS-PAGE, the correct answer is:
(d) All of the above ✅
Soniya Shekhawat
August 14, 2025All of the above.
Roopal Sharma
August 14, 2025Bromophenol blue ,orange g, xylene cynol all are used for tracking
Priya dhakad
August 14, 2025Answer is d
All three dyes are used as tracking dye in SDS PHAGE
Mansukh Kapoor
August 14, 2025The correct answer is option 4th
All the dyes are used in tracking of the sample in gel
Mohd juber Ali
August 14, 2025Option d is the right answer
Deepika Sheoran
August 14, 2025Option 4th is correct answer
Because all three dyes can be used as tracking dyes in SDS PAGE.
Dipti Sharma
August 14, 2025(d) All of the above
HIMANI FAUJDAR
August 14, 2025All of these are used as tracking dye
Sneha Kumawat
August 14, 2025All of the above
Surbhi Rajawat
August 14, 2025Understood.
Priyanka choudhary
August 14, 2025All off above
Shivani
August 14, 2025All of the above .
Priya khandal
August 14, 2025All of the above
Kajal
August 15, 2025All of these
shruti sharma
August 16, 2025all of these
Kavita choudhary
August 17, 2025All of the above
Aafreen
August 17, 2025Ans – 4 All of the Above
Avni
August 17, 2025All of the above
Neeraj Sharma
August 17, 2025Option D is correct
Simran Saini
August 18, 2025All of the above.
Varsha Tatla
August 23, 2025Bromo phenol , orange g etc all of these
Bharti Yadav
August 24, 2025Bromophenol blue, xylene cyanol,orange G all three dyes can be used as tracking dyes in SDS -PAGE
Priyanka Choudhary
August 24, 2025Sds page of protein,
Bromo phenol blue,Xylene cyanol, Orange G
MOHIT AKHAND
August 24, 2025D
Done sir ✅
Urmi sisodiya
August 24, 2025All of the above
Anil Choudhary
August 24, 2025All
Bhavana kankhedia
August 24, 2025All of the above
Alec
August 24, 2025all of the above dyes are used.
Sonam Saini
August 24, 2025Option d is right answer
Niti Tanwar
August 24, 2025All of the above
Anjali
August 24, 2025All of the above
Anju
August 24, 2025All of the above
Seema
August 24, 2025D) all of these dyes are used.
Shivani Panwar
August 24, 2025All of the above….
Devika
August 24, 2025All of these
Falguni Prakash
August 24, 2025All of them
Kashish yadav
August 25, 2025Ok sir 👍
Ayush Dubey
August 25, 2025All of the above
sakshi khatri
August 25, 2025done
Pratibha Jain
August 30, 2025The correct answer is option (d)
All of the above
Shriyanshi verma
August 30, 2025Bpb, xylene cyanol, orang g
Surendra Doodi
August 30, 2025Bromophenol Blue ,Xylene Cyanol, orange G all
Patel Radha
August 30, 2025Done ✔️
Komal Sharma
August 31, 2025In SDS-PAGE (Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis), tracking dyes are used to monitor the progress of electrophoresis and ensure proper migration of proteins. Different dyes migrate at different speeds, allowing flexibility based on experimental needs.
Vanshika Sharma
August 31, 2025All of the above dyes used in SDS
Shobha Kanwar
August 31, 2025All of these :Bromophenol blue ,orange g, xylene cynol all are used for tracking
Aartii sharma
September 1, 2025All of these are used as dye
Anisha jakhar
September 3, 2025The correct option is (d) All of the above
Muskan Yadav
September 3, 2025All of these are used as dye ( bromophenol blue , xylene cyanol and orange g )
Somya Yadav
September 5, 2025All of the above
Ajay Sharma
September 6, 2025All of the above
Meera Gurjar
September 13, 2025All of them
Aakansha sharma Sharma
October 1, 2025Bromophenol blue ,orange g, xylene cynol all are used for tracking