Q.19 Which one of the following techniques/tools is NOT used for inserting a foreign
gene into a cell?
(A) DNA microarray
(B) Electroporation
(C) Gene gun
(D) Microinjection
Which Technique Is Not Used for Inserting Foreign Genes? Unraveling DNA Microarray vs. Electroporation, Gene Gun, Microinjection
DNA microarray stands out as the technique not used for inserting foreign genes into cells, unlike electroporation, gene gun, and microinjection, which are established gene delivery methods in molecular biology.
Correct Answer
(A) DNA microarray is the option that does not serve as a tool for inserting foreign genes into cells.
Option Breakdown
DNA Microarray
DNA microarray involves microscopic DNA spots on a solid surface for analyzing gene expression through hybridization of labeled samples, not for delivering genes into cells. It detects expression levels by binding complementary sequences but lacks mechanisms to penetrate cell membranes.
Electroporation
Electroporation applies electric pulses to create temporary pores in cell membranes, allowing foreign DNA to enter efficiently, especially in bacteria, yeast, and plant protoplasts.
Gene Gun
The gene gun, or biolistic method, propels DNA-coated gold or tungsten particles into cells using high-velocity mechanical force, bypassing cell walls in plants and organelles.
Microinjection
Microinjection uses a fine glass needle to directly inject foreign DNA into the cell nucleus or cytoplasm, offering precision for single cells like animal oocytes or plant protoplasts.
