A protein solution (0.2 ml) of unknown concentration was diluted with 0.8 ml of water. To 0.5 ml of this diluted solution 4.5 ml of biuret reagent was added and […]
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CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Understanding the Units of Molar Extinction Coefficient (ε)
The units of molar extinction coefficient are (1) L mol-1 crn-1 (2) cm-1 mg ml-1 (3) mol-1 mm (4) mol cm ml-1 Correct Answer: (1) L mol⁻¹ cm⁻¹ Topic Explanation: […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Calculating Protein Concentration Using UV Absorbance and Molar Extinction Coefficients
A protein has one tryptophan and one, tyrosine in its sequence. Assume molar extinction coefficients at 280 nm of tryptophan and tyrosine as 3000 and 1500 M-1cm-l, respectively. What would […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Relationship Between Absorbance and Transmission in Spectrophotometry
What will be the percentage transmission when absorbance is 1, 2 and 3, respectively? (1) 10, 1, 0.1 (2) 1, 10, 100, (3) 0.2, 0.1, 0 (4) 20, 10, 0 […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
UV Spectroscopy for most sensitive label-free Protein Quantification
Which one of the methods listed below is the most sensitive label-free quantification method for proteins (1) UV spectroscopy (2) Infra-red spectroscopy (3) Raman spectroscopy (4) 13C content of protein […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Intrinsic Fluorescence of Protein Is Due to Aromatic amino acids Tryptophan Tyrosine and Phenylalanine
Which one of the following would contribute to intrinsic fluorescence to a protein? (1) aromatic amino acids (2) disulfide bonds (3) charged amino acids (4) branched chain amino acids […]
CSIR NET LIFE SCIENCE FREE TEST DEVELOPMENTAL BIOLOGY
CSIR NET Life Science Free Test on Morphogenesis and Organogenesis in Plants
CSIR NET Life Science Free Test on Morphogenesis and Organogenesis in Plants by Let’s Talk Academy Master Plant Development with a Free CSIR NET Life Science Test (Unit 5 […]
CSIR NET LIFE SCIENCE FREE TEST DEVELOPMENTAL BIOLOGY
CSIR NET Life Science Free Test on Morphogenesis and Organogenesis in Animals
CSIR NET Life Science Free Test on Morphogenesis and Organogenesis in Animals by Let’s Talk Academy Introduction: Let Morphogenesis and Organogenesis Make or Break Your CSIR NET Rank When […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Relationship Among RNA Polymerase II Subunits IIo, IIa, and IIb
Which one of the following schematics depicts the potential relationship among the subunits IIo, IIa, and IIb of RNA polymerase II Relationship Among RNA Polymerase II Subunits IIo, IIa, and […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Regulatory Elements for Expression of Ribosomal RNA Genes in Eukaryotes
27. Regulatory elements for expression of ribosomal RNA genes resides in the (1) transcribed spacer region (2) non-transcribe spacer region (3) 5’ flanking region of individual ribosomal genes (4) internal […]
