In a genetic assay, randomly generated fragments of yeast DNA were cloned into a bacterial plasmid containing gene ‘X’ essential for yeast viability on minimal media. The recombinant plasmid was […]
Tag: csir pyq molecular biology
Tag: csir pyq molecular biology
csir pyq molecular biology
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Minimum Number of tRNAs Required to Decode All Arginine Codons Without Inosine
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- June 14, 2025
- 7 Comments
The amino acid arginine is encoded by six codon: CGU, CGC, CGA, CGG, AGA, and AGG. Assuming inosine is not an option in the tRNA anticodon, what is the minimum […]
Key Steps in Eukaryotic DNA Replication That Require ATP Hydrolysis
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- June 14, 2025
- 42 Comments
Some of the steps in the process of eukaryotic DNA replication mentioned below require hydrolysis of ATP. (A) Phosphodiester bond formation (B) DNA strand separation by helicase (C) Clamp-loader association […]
What Is a Polysome? The Role of Multiple Ribosomes on a Single mRNA Strand
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- June 14, 2025
- 10 Comments
The polysome can be describes as (1) A special ribosome occurring in prokaryotes (2) A DNA strand which is being transcribed by may RNA polymerase(3) String of RNA occupied by […]
What Happens to Sliding Clamp-Loaders After They Load Sliding Clamps Onto DNA?
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- June 14, 2025
- 20 Comments
The high processivity of DNA polymerases during replication is because of their association with proteins called sliding clamps. For loading onto DNA, sliding clamps require sliding clamp-loaders. Which of the […]
Which Polypeptide Cannot Be Produced from a Given DNA Sequence in Any Reading Frame?
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- June 14, 2025
- 5 Comments
Given below is a partial coding sequence of a gene: 5′.AATGGACGCATGTGTCGATGG-3′ Which one of the following polypeptides CANNOT be produced by transcription and translation of the above DNA sequence in […]
Understanding Replication Forks, Bubbles, and DNA Synthesis According to Okazaki
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- June 14, 2025
- 30 Comments
65. The following statements are made on DNA replication: A. Replication fork is a branch point in a replication ‘eye’ or ‘bubble’. B. A replication bubble contains two replication forks. […]
Differences in Peptide Translation of the Same RNA Sequence in Mammalian Cytosol and Mitochondria
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- June 14, 2025
- 4 Comments
Imagine the following RNA sequence is translated in the mammalian cytosol and mitochondria. RNA sequence: AUG AUA CUG UGA CUU AGG CUC UAA Following are some putative peptide sequences CytosolMitochondria […]
Key Facts About Eukaryotic DNA Replication: Origins, Pulse-Chase Labeling, and Elongation Rates
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- June 14, 2025
- 11 Comments
Following are certain statements related to eukaryotic DNA replication: The genome of multicellular animals contain many potential origins of replication. During early development, when embryos are undergoing rapid cell divisions, […]
How to Translate DNA Sequence 3’-AAGTACTCT-5’ into a Tripeptide: Phe-Met-Arg Explained
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- June 14, 2025
- 9 Comments
What would be the tripeptide produced by translation of the transcript produced by the following DNA sequence? 3’-AAGTACTCT-5’ (1) Arg-Phe-Trp (2) Arg – Leu – Gly (3) Thr- Lys —Ser […]


