38. In a mixture of the five proteins listed below, which should elute second in size-exclusion (gel-filtration) chromatography? A. Cytochrome c (Mw = 13,000) B. Immunoglobulin G (Mw = 145,000) […]
Tag: CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY
No posts found.
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Methods to Check Molecular Weight
37. Which of the following methods could be used to check the molecular weight of your purified protein? A. SDS-PAGE only B. Mass spectrometry only C. Analytical size exclusion chromatography […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Elute Target Proteins from Affinity Chromatography Matrix
36. To elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate? A. Low salt concentrations B. High salt concentrations C. Adding […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Hydrophobic Interaction Chromatography
35. What properties of a protein does hydrophobic interaction chromatography exploit for purification? A. Charged amino acids B. Hydrophobic amino acids on the protein surface C. Molecular weight D. Enzyme […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Best Protein Purification Strategy
34. For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Back Diffusion Gas Chromatography
33. Back diffusion occurs mostly in: A. Size exclusion chromatography B. Gas chromatography C. HPLC D. HPTLC Back diffusion occurs mostly in Gas Chromatography (GC), making option (B) correct. This is due […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
GC HPLC Stationary Phase Bleed
32. The process of release of volatile / soluble components of a GC or HPLC stationary phase is termed as: A. Bleed B. Creep C. Back flush D. Back diffusion […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Theoretical Plates Chromatography
31. Which of the following is the correct statement: A. Resolution is proportional to the square root of the number of theoretical plates in a column B. Resolution is inversely […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
α-Bungarotoxin Binds Acetylcholine Receptor
30. α-bungarotoxin binds to acetylcholine receptor (AChR) protein with high specificity and prevents the ion channel opening. This interaction can be exploited to purify AChR from membrane using (A) Ion-exchange […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Chromatography Solid Stationary Phase
29. Chromatography with solid stationary phase is called (A) Circle chromatography (B) Square chromatography (C) Solid chromatography Chromatography with solid stationary phase is called adsorption chromatography. The correct answer is […]
