77. During DNA replication the newly generated strand remains attached to the template. However, during
transcription newly synthesized RNA dissociates from the template. This is achieved by:
1. RNase H
2. RNA polymerase itself
3. DNA Polymerase
4. Spontaneous dissociation

Detailed Explanation:

During DNA replication, the newly synthesized strand remains attached to the template strand. However, during transcription, the RNA polymerase synthesizes RNA, which then dissociates from the DNA template. This dissociation is essential for proper transcription and gene expression. It occurs because RNA polymerase itself facilitates this process through conformational changes and the release of RNA from the DNA template.

Thus, the correct answer is:
2. RNA polymerase itself