Reverse Transcription Polymerase Chain Reaction
Why would a researcher want to use RT-PCR?
a. RT-PCR is used to compare two different samples of DNA for relatedness.
b. RT-PCR creates an mRNA molecule from a known DNA sequence.
c. RT-PCR generates a protein sequence from mRNA.
d. RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Correct Answer: (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
🔎 What is RT-PCR?
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a technique used to convert RNA into complementary DNA (cDNA) and then amplify specific DNA sequences. It is widely used to analyze gene expression, detect RNA viruses, and produce cDNA from mature mRNA transcripts.
🌟 1. How RT-PCR Works
✅ Step 1: Reverse Transcription
- RNA is extracted from the sample.
- A reverse transcriptase enzyme is used to synthesize complementary DNA (cDNA) from the RNA template.
✅ Step 2: PCR Amplification
- The cDNA is amplified using specific primers and a DNA polymerase.
- This allows for the detection and quantification of specific RNA transcripts.
✅ Step 3: Gel Electrophoresis or Real-Time Monitoring
- The amplified products are analyzed through gel electrophoresis or fluorescence-based detection.
🧬 2. Why RT-PCR is Useful
- RNA molecules (like mRNA) are unstable and difficult to analyze directly.
- Converting RNA into cDNA allows researchers to amplify, analyze, and quantify gene expression.
- cDNA generated from mRNA lacks introns because only exons are present in mature mRNA, making RT-PCR valuable for studying gene expression.
✅ 3. Why the Other Options Are Incorrect
| Option | Explanation | True/False |
|---|---|---|
| (a) RT-PCR is used to compare two different samples of DNA for relatedness. | DNA relatedness is analyzed using techniques like RAPD or RFLP, not RT-PCR. | ❌ False |
| (b) RT-PCR creates an mRNA molecule from a known DNA sequence. | RT-PCR works in the opposite direction — it creates cDNA from RNA, not RNA from DNA. | ❌ False |
| (c) RT-PCR generates a protein sequence from mRNA. | Translation (not RT-PCR) is responsible for protein synthesis from mRNA. | ❌ False |
| (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA. | RT-PCR creates cDNA from processed mRNA, which lacks introns. | ✅ True |
🧪 4. Applications of RT-PCR
✅ 1. Gene Expression Analysis
- Measures the expression level of specific genes.
- Used to compare gene activity under different experimental conditions.
✅ 2. Viral Detection
- Detects RNA viruses like HIV, SARS-CoV-2 (COVID-19), and influenza.
- Amplifies viral RNA to enable easy detection.
✅ 3. Cloning and Sequencing
- Produces cDNA for cloning into vectors for further analysis.
- Used to analyze transcriptomes (total RNA in a cell).
✅ 4. Genetic Mutation Studies
- Identifies alternative splicing events or mutations in mRNA.
- Helps in understanding genetic diseases.
🌡️ 5. How RT-PCR Differs from Other PCR Techniques
| Technique | Purpose |
|---|---|
| Standard PCR | Amplify known DNA sequences. |
| RT-PCR | Convert RNA into cDNA and amplify it. |
| qPCR (Quantitative PCR) | Quantify the amount of DNA amplified in real time. |
| RAPD (Random Amplified Polymorphic DNA) | Assess genetic diversity using random primers. |
| Inverse PCR | Amplify DNA regions flanking a known sequence. |
🚀 6. Why RT-PCR is Important
✅ Fast and reliable method to detect RNA-based pathogens.
✅ Quantifies gene expression levels accurately.
✅ Essential tool for molecular diagnostics and biotechnology.
✅ Helps distinguish between coding and noncoding regions of genes.
🔬 7. Limitations of RT-PCR
❌ RNA Degradation – RNA is more fragile than DNA and prone to degradation.
❌ Primer Design Sensitivity – Poor primer design can lead to non-specific amplification.
❌ Reverse Transcriptase Errors – Reverse transcriptase lacks proofreading ability, leading to errors.
📌 Summary
- RT-PCR converts mRNA into cDNA, allowing analysis of gene expression and RNA viruses.
- cDNA produced through RT-PCR lacks introns since mature mRNA contains only exons.
- RT-PCR is widely used in molecular diagnostics, virology, and genetic research.
- ✅ Correct Answer: (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
78 Comments
Akshay mahawar
March 17, 2025Done i
Nisha
March 17, 2025Done
Mahima Sharma
August 24, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Nisha
March 17, 2025Good explanation
Arushi
March 17, 2025👍👍
Suman bhakar
March 20, 2025👍👍
Parul
March 23, 2025Easily done sir.
Ujjwal
March 26, 2025✔️👍
Soniya Shekhawat
August 24, 2025In RT PCR DNA is amplifier but the source is mrna.
Khushi Pareek
August 24, 2025Rt PCR: mRNA to DNA by using reverse transcriptase and mRNA is intron free
Mohd juber Ali
August 24, 20251st step of RT PCR is conversion of mRna into dna
Sakshi yadav
August 24, 2025In Rt pcr the DNA is amplified but the source of DNA is mrna
Arushi Saini
August 24, 2025CDNA is generated in rt pcr
Mansukh Kapoor
August 24, 2025The correct answer is option 4th
RT-PCR generates a DNA molecule without the introns from eukaryotic mRNA
Meenakshi Choudhary
August 24, 2025Option d is correct
Avni
August 24, 2025Correct Answer: (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Neha Yadav
August 24, 2025Researcher want to use RT – PCR because it covert RNA into cDNA
Roopal Sharma
August 24, 2025RT PCR creates cdna from mrna . mrna is freee from exons .
karishma don
August 24, 2025RT-PCR generates a DNA from mRNA.
Khushi Agarwal
August 24, 2025Option d is correct
RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA
Anurag Giri
August 24, 2025Option 4th
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a technique used to convert RNA into complementary DNA (cDNA) and then amplify specific DNA sequences
Dharmpal Swami
August 24, 2025RT -PCR generate a DNA from eukaryotic mRNA
Aakanksha Sharma
August 24, 2025Rt-pcr generate a dna from m- rna
Aafreen Khan
August 24, 2025RT-PCR is a technique to convert RNA into complementary DNA and then amplify specific DNA sequence. RT-PCR generates a DNA molecule without the non coding introns from eukaryotic mRNA
Karishma
August 24, 2025Rt pcr is converted 1 ran in to 1 dna
Karishma
August 24, 2025Rt pcr is converted 1 ran in to 1 dna option d correct answer
Priyanka Choudhary
August 24, 2025Option d is correct,
RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA
Dipti Sharma
August 24, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
mRna to Dna
HIMANI FAUJDAR
August 24, 2025Ans RT PCR generated DNA molecules without using any non coding intron.
Priya dhakad
August 24, 2025RT-PCR generates a DNA molecule without the non coding introns from eukaryotic mRNA.
AKANKSHA RAJPUT
August 24, 2025In Rt pcr the DNA is amplified but the source of DNA is mrna
anjani sharma
August 24, 2025RT-PCR creates cDNA from processed mRNA, which lacks introns.
Answer d
Tanvi Panwar
August 24, 2025RT-PCR produces DNA without noncoding introns from the mRNA
Heena Mahlawat
August 24, 2025Rt pcr is used to produce cdna from mrna
Sneha Kumawat
August 24, 2025Rt pcr generate at DNA molecule without the non coding interference from eukaryotic mrna
MOHIT AKHAND
August 24, 2025Done sir ✅
Ayush Dubey
August 24, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Santosh Saini
August 24, 2025Through the RT PCR tech. mRNA convert into complementary DNA (cDNA) without non coding introns
Neelam Sharma
August 24, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA. Because RT PCR create dna form mRNA it has lack intron
Bharti Yadav
August 24, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Bhawna Choudhary
August 24, 2025Option D is correct
Priyanka Verma
August 24, 2025Option D is correct – RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Monika jangid
August 24, 2025Option d bcoz the rtpcr amplify the dna from mrna
Varsha Tatla
August 25, 2025It creates cDNA from rna and amplify it
Divya rani
August 25, 2025RTPCR is the technique in which mrna converted into cDNA without noncoding introns from eukaryotic mrna.
Aman Choudhary
August 25, 2025In RT-PCR conversion from m-RNA to DNA
shruti sharma
August 25, 2025RT PCR is the technique in which mrna converted into cDNA without non coding introns from
Komal Sharma
August 25, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Payal Gaur
August 25, 2025In RT -pcr firstly mRNA convert into DNA and DNA amplified by using 1st specific primer which are design from 5′ end of
m RNA and N terminal of protein and 2nd primer used (common primer) oligo DT primer and gene amplified by RT pcr
Bhavana kankhedia
August 26, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Surendra Doodi
August 26, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA. Best Institute for CSIR NET Life Science Coaching – Let’s Talk Academy”
Vanshika Sharma
August 26, 2025RT pcr generates a dna molecule without a non coding introns from euk mrna
Minal Sethi
August 26, 2025In RT-PCR the source of DNA is m-RNA and then the DNA is amplified by PCR
option D
Shivani
August 26, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Rishita
August 26, 2025Okey sir done 👍🏻
Manisha
August 26, 2025Done ✅
Alec
August 26, 2025Rt-pcr generates the dna molecule without non coding introns from eukaryotic mrna.
Devika
August 26, 2025Option D
Simran Saini
August 26, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Samiksha bajiya
August 27, 2025Option D is correct
RT PCR by using reverse transcriptase enzyme convert RNA into complimentary dna
Seema
August 27, 2025Rt-pcr means dna from m-RNA and amplification of dna .
Rakesh Dhaka
August 27, 2025Option d is correct
In RT-PCR conversion from m-RNA to DNA
Sakshi Kanwar
August 27, 2025used to convert RNA into DNA and then amplify specific DNA with the activity of reverse transcriptase
Mitali saini
August 28, 2025Correct Answer: (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Muskan singodiya
August 28, 2025Rt-pcr the dna is amplify but the source of dna is mrna
Kanica Sunwalka
August 28, 2025done
Nilofar Khan
August 28, 2025Option d is correct
d.RT-PCR generates a DNA molecule without the introns from eukaryotic mRNA
Deepika Sheoran
August 28, 2025Option D is correct
RT PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA..
Kajal
August 28, 2025Option d is correct as RT -PCR is used to generate dna molecule with out the non coding intron from eukaryotic dna ..
Anisha Beniwal
August 28, 2025Option D is correct
Neeraj Sharma
August 28, 2025In rtpcr the mrna is first converted into DNA and then DNA is amplified
Khushi Vaishnav
August 28, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Parul Yadav
August 29, 2025RT-PCR generates the DNA without the non-coding introns from the mRNA.
Khushi Singh
August 30, 2025RT-PCR generates dna molecule without non coding introns from eukaryotic mRNa
Sonam Saini
August 30, 2025RT-PCR Eukaryotic from m rna intron ki bina DNA utpann karta hai
Kajal
August 31, 2025Correct Answer is (d) RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA
Konika Naval
August 31, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.
Muskan Yadav
September 8, 2025RT-PCR generates a DNA molecule without the noncoding introns from eukaryotic mRNA.