An observation was being made under a compound microscope using light of 500 nm wave length. The refractive index of the observation medium was 1.5. The Sinα of the lens […]
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CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Oil Immersion Microscopy: Resolving Power Numerical Aperture, and Light Refraction
In an oil immersion microscope, a drop of oil must be placed between the tip of objective lens and the specimen. The oil A. reduces the numerical aperture of the […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Improving the Resolution of a Microscope
The most important property of any microscope is its resolution (D) and can be calculated from the formula D = where D is, minimum distance between two distinguishable objects, A. […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
Microscope Resolution | Parameters Affecting Resolution Wavelength | Refractive Index | Angular Aperture
The most important property of any microscope is its power of resolution, which is numerically equivalent to D; the minimum distance between two distinguishable objects. D depends on three parameters […]
CSIR NET Life Science Previous Year Questions on METHODS IN BIOLOGY – Video and Text Solutions
The Resolving Power of a Bright Field Microscope
Which one of the following factors will NOT have any impact on the resolving power of a bright field microscope? (1) Color of light (2) Intensity of light (3) Angle […]
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Gate Life Science Online Offline Coaching
The Graduate Aptitude Test in Engineering (GATE) in Life Science (XL) is a well-known test that gives students a lot of options for graduate school and research at India’s best […]
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Gate biotechnology Online Offline Coaching
The Graduate Aptitude Test in Engineering (GATE) is a huge deal for science and technology graduates all throughout India. The Indian Institute of Science (IISc) and seven Indian Institutes of […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Calculation of Protein Concentration Using the Biuret Assay
A protein solution (0.2 ml) of unknown concentration was diluted with 0.8 ml of water. To 0.5 ml of this diluted solution 4.5 ml of biuret reagent was added and […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Understanding the Units of Molar Extinction Coefficient (ε)
The units of molar extinction coefficient are (1) L mol-1 crn-1 (2) cm-1 mg ml-1 (3) mol-1 mm (4) mol cm ml-1 Correct Answer: (1) L mol⁻¹ cm⁻¹ Topic Explanation: […]
CSIR NET Life Science Previous Year Questions and Solutions Spectroscopy
Calculating Protein Concentration Using UV Absorbance and Molar Extinction Coefficients
A protein has one tryptophan and one, tyrosine in its sequence. Assume molar extinction coefficients at 280 nm of tryptophan and tyrosine as 3000 and 1500 M-1cm-l, respectively. What would […]
