Tag: GATE Life Science Previous Year Papers with Solutions

Q.42 Which of the four restriction enzymes given below cut the following DNA sequence? 5’-CCGATATCTCGAGGGC-3’ (P) BamH1 (3’-CCTAG^G-5’) (Q) XhoI (3’-GAGCT^C-5’) (R) EcoRI (3’-CTTAA^G-5’) (S) EcoRV (3’-CTA^TAG-5’) (A) P & […]

Q.41 A researcher wants to clone 3 DNA fragments of sizes 1.1 Mb, 0.097 Mb and 0.045 Mb. The choice of the vectors for cloning each of the fragments are: (A) […]

Q.40 Match the following enzymes with their regulatory mechanism (a) Phosphofructokinase                       1. Product inhibition (b) Glycogen synthase        […]

Q.39 Arrange the following in the increasing order of amount of ATP generated by metabolism of one molecule of the following compounds. (i) Anaerobic catabolism of starch with 300 glucose […]

Q.38 Arrange the following in the decreasing order of their permeability coefficients across a lipid bilayer membrane. (i) Urea (ii) Glucose (iii) H2O (iv) Na+ (v) Tryptophan (A) (i), (iii), […]

Q.37 The following figures show the plot of reaction rate (v) versus substrate concentration (mM) for an enzyme-catalyzed reaction in the presence and absence of an inhibitor. Match the possible reaction […]

Q.36 You have prepared 1.0 liter of 0.5 M acetate buffer (pH = 5.0). The dissociation constant of acetic acid is 1.7 × 10−5 M. What would be the acetate ion […]

Q.35 In different types of tissue transplantations, the rate of graft rejection in decreasing order is (A) Isograft > Xenograft > Allograft (B) Allograft > Isograft > Xenograft (C) Xenograft […]

Q.34 An oligonucleotide was sequenced by the dideoxy method of Sanger and the following autoradiogram was obtained The sequence of the oligonucleotide is (A) 3’-GTCCTGTACA-5’ (B) 5’-GTCCTGTACA-3’ (C) 5’-ACATGTCCTG-3’ (D) […]

Q.33 An enzyme has two binding sites for an inhibitor molecule. When the inhibitor binds to the first site, the dissociation constant of the inhibitor for the second site increases, […]