Interpreting Hemoglobin Electrophoresis and Western Blot Results for Sickle Cell Disease You order a hemoglobin electrophoresis on a patient suspected of having sickle cell disease. A blood sample was obtained […]
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CSIR NET Previous Year Questions on Blotting
Best Technique to Study Protein Half-Life in Animal Cells
Best Technique to Study Protein Half-Life in Animal Cells Which of the following techniques should be used to study the half-life of protein A in an animal cell that can […]
CSIR NET Previous Year Questions on Blotting
Limitations of Northern Blotting
Limitations of Northern Blotting or Which of the following analysis cant be done using Northern blotting A. Approximate size of an mRNA B. Relative abundance of an mRNA in various […]
CSIR NET Previous Year Questions on Blotting
Interpreting Southern Blot Results
Interpreting Southern Blot Results Isolated variety of E .coli is followed by southern blot procedure. A radio active probe is then hybridized with gene of interest & observed single band […]
CSIR NET Previous Year Questions on Blotting
Technique to Locate Developmental RNAs
1. Which of the following technique is most suitable to demonstrate that particular RNAs that are important for development are located in distinct regions off the Drosophila embryo ? […]
CSIR NET Previous Year Questions Gel Electrophoresis
Cytochrome-C and Myoglobin separated by polyacrylamide gel electrophoresis
A mixture of Cytochrome-C (MW 11.7 kD) and Myoglobin (MW 17.2 KD) are to be separated by polyacrylamide gel electrophoresis. Their isoelectric pH (pI) values are 9.6 and 7.2 respectively. […]
CSIR NET Previous Year Questions Gel Electrophoresis
Determination of the molecular weight by molecular exclusion chromatography
A protein was purified to homogeneity. Determination of the molecular weight by molecular exclusion chromatography yields 60 kd. Chromatography in the presence of 6 M urea yields a 30-kd species. […]
CSIR NET Previous Year Questions Gel Electrophoresis
DNA replication with radio-labeled nucleotides, followed by urea-polyacrylamide gel electrophoresis (PAGE)
In an invitro experiment using radio-labeled nucleotides, a researcher is trying to analyze the possible products or DNA replication by resolving the products using urea-polyacrylamide gel electrophoresis. In one experimental […]
CSIR NET Previous Year Questions Gel Electrophoresis
To determine the Isoelectric point of a protein
To determine the isoelectric point of a protein, first establish that a gel: (a) Contains a denaturing detergent that can distribute uniform negative charges over the protein’s surface. (b) Exhibits […]
CSIR NET Previous Year Questions Gel Electrophoresis
Two-dimensional gel electrophoresis (2D-GE)
The first step in two-dimensional gel electrophoresis generates a series of protein bands by isoelectric focusing. In a second step a strip of this gel is turned 90 degrees, placed […]
