When one isolates ribosomes from bacterial lysate, apart from 70S. 50S and 30S ribosomal subunits. One also finds a small population of 100S, 130S and 150S sub-units. EDTA dissociates these […]
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Understanding Eukaryotic Gene Regulation: Key Statements and Their Accuracy
4. Following statements were made about regulation of eukaryotic gene expression. A. It is usually regulated at the level of initiation of altering the chromatin by transcription architecture. B. The […]
How to Interpret Polysome Profiles for CHP, LTM, and PTM Translation Inhibitors
11. Polysome profiling of cells treated with three hypothetical translation inhibitors is shown in the plot below. These three inhibitors are (i) CHP — leaky inhibitor of translation (ii) LTM […]
Interpreting Pulse-Chase Experiments: Can Protein Dynamics Reveal Causal Relationships?
3. In response to a drug, changes in protein levels wereexamined in a cell line. A pulse chase experiment wasperformed using labeled methionine. In comparison, to untreated samples, the following […]
Cycloheximide: The Antibiotic That Specifically Inhibits the Eukaryotic 80S Ribosome
Among the following which antibiotic is inhibitor of 80S ribosome? (1) Tetracycline (2) Streptomycin (3) Cycloheximide(4) Chloramphenicol Antibiotics are widely known for their ability to inhibit bacterial protein synthesis by […]
GAL Gene Regulation in Yeast: Identifying the False Statement
Which one of the following statements about GAL gene expression is FALSE? (1) GALA is a positive regulator of GAL genes (2) The UAS region that regulates the GAL geneexpression […]
Erythromycin Inhibits Bacterial Protein Synthesis by Blocking Elongation After Initial Codons Are Translated
Which one of the following statements is correct in the context of erythromycin-mediated inhibition of protein synthesis in bacteria? (1) Erythromycin inhibits protein synthesis at the step of elongation, but […]
Matching Transcription Factors to Their DNA-Binding Domains: A Guide to Protein-DNA Recognition
1. Which of the options correctly matches the proteins involved in transcription (Column A ) with the DNA they carry ( Column B) Column A Column B A. TFIIIA i. […]
Which one of the following statements is correct in the context of erythromycin-mediated inhibition of protein synthesis in bacteria? (1) Erythromycin inhibits protein synthesis at the step of elongation, but […]
How Erythromycin Inhibits Bacterial Protein Synthesis: Binding to the 50S Ribosomal Subunit
8. Erythromycin is an inhibitor of protein synthesis. It acts by: (1) binding to 30S subunit of bacterial ribosome, thus inhibiting binding of aminoacyl-tRNAs. (2) binding to 50S subunit of […]


