7. Tetracycline’s are a group of broad-spectrum antibiotics against bacterial resistance. Tetracycline antibiotics are protein synthesis inhibitors and exerts its effect by binding to (1) 30 S subunit of ribosome […]
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Puromycin: The Antibiotic That Mimics the 3′ End of Charged tRNA
6.An antibiotic that resembles the 3′ end of a charged tRNA molecule is: (1) Streptomycin(2) Sporsomycin (3) Puromycin (4) Tetracycline Antibiotics have revolutionized medicine and molecular biology by targeting critical […]
Bacterial Transcriptional Regulation Mechanisms to Operons and Processes
42. Contents in Column I and II are with respect to bacterial transcriptional regulation. Column I Column II i. Positive regulation by Camp a. Ara operon ii. Short abortive b. […]
6.An antibiotic that resembles the 3′ end of a charged tRNA molecule is: (1) Streptomycin(2) Sporsomycin (3) Puromycin (4) Tetracycline
Puromycin Mechanism of Action: True Statements and Biological Insights
5. Puromycin is an antibiotic used to inhibit protein synthesis. Given below are few statements about the antibiotic. A. It enters the E-site of the ribosome where it prevents the […]
Understanding trp-lac Fusion Operon Behavior: Regulation and Expression in E. coli
41. In a strain of E.coli, a fusion between the lac and trp operon took place and the new locus structure is shown below. The strain lacks the wild type […]
How Puromycin Blocks Translation: Mechanism, Molecular Action, and Biological Significance
4. Puromycin blocks translation. Mode of action of drug puromycin is (1) binds to A site and leads to premature termination (2) stops peptidyl transferase activity (3) Binds EF-TU-GTP and […]
Transcriptional Regulation of the trp Operon by Tryptophan: Mechanism and Biological Significance
40. Transcriptional regulation of trp operon by tryptophan involves binding of tryptophan to (1) the repressor protein and inhibition of transcription by its interaction with the operator region. (2) RNA […]
Which Antibiotic Causes Premature Termination of Translation in Bacteria? Mechanism, Uses, and Insights
3. Which antibiotic is responsible for premature termination of translation in bacteria? (1) Tetracycline (2) Choloramphenicol (3) Penicillin (4) Puromycin Translation is a fundamental biological process in all living cells, […]
Bidirectional Promoter Regulation in E. coli: How Loss of Regulatory Protein Binding Alters Reporter Gene Activity
39. A construct (shown below) was generated to access the activity of bidirectional promotor (A and B) which has a common regulatory DNA element (X). The construct was used to […]


