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17. Which of following is reactive centre for splicing of exons during processing of m-RNA? (1) U1 and U5 (2) Branch point, U2 and U6 (3) Branch point, U4 and […]

16. A 1257 bp genomic DNA sequence of a prokaryotic gene was cloned under a strong constitutive promoter along with a suitable polyA signal and used for development of transgenic […]

15. Regarding gene expression of eukaryotes, which statement is correct? (1) m-RNA and template DNA are has same sequence (2) m-RNA and protein synthesis can occur simultaneously (3) processing of […]

The Role of Cyclin T1 in the Human P-TEFb Complex: Key Regulator of Transcription Elongation 14. The positive transcription elongation factor. P-TEFb, is a Mufti-protein complex that plays an essential […]

13. Which one of the following statements about eukaryotic RNA processing is INCORRECT? (1) Termination of transcription occurs co- transcriptionally at the polyadenylation site. (2) Phosphorylation of the Ser 2 […]

12. The 3’ end of most eukaryotic mRNAs is defined by the addition of a polyA tail- a processing reaction called polyadenylation. The addition of poly A tail is carried […]

11. You are working with an in vitro eukaryotic transcription system, which produced both capped and uncapped mRNAs. You incubated these mRNAs with mammalian cell nuclear extract and then quantified […]

Introns in Eukaryotic Genes: Found in rRNA, tRNA, and mRNA Encoding Genes 10. Introns in the eukaryotic genes are found in: (1) rRNA and mRNA encoding genes but not in […]

9. A gene consists of two introns, a 5′ UTR and 3’UTR region, the probable number of exon will be. (1) 2             (2) 3 (3) 4             (4) 5 Calculating the […]

8. An eukaryotic cell undergoing mRNA synthesis and processing was incubated with32P labelled ATP, with the label at the β-position. Where do you think the radioactive isotope will appear in […]