27. Following statements were made about the post-transcriptional processing of RNA in eukaryotes. A. Soon after transcription initiation, RNA polymerase II pauses -30 nucleotides downstream from the site of initiation […]
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CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Transcription and Processing of mRNA | Nonsense-Mediated Decay
26. Which one of the following statements related to transcription and processing of mRNA is INCORRECT? (1) During prokaryotic transcription, DNA binding properties of RNA polymerase are altered by sigma […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
The Formation of 2′-5′Phosphodiester Bonds During RNA Maturation
25. During maturation process of some RNA molecules, formation of a 2′-5′ phospho-diester bond takes place. This Following statements are made about phenomenon. A. Spliceosome mediated removal of intronic sequences […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Splicing of pre-mRNA is mediated by snRNAs
24. During m-RNA processing splicing is mediated by- (1) sn RNA (2) sno-RNA (3) r-RNA (4) t-RNA The correct answer is (1) snRNA. Explanation: During pre-mRNA processing, splicing is mediated by the spliceosome, […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
The snRNA that base pairs with the 5′ splice site of pre-mRNA is U1 snRNA
23. Which one of the snRNAs given below base pairs with 5’splice Site of pre mRNA? (1) U1 (2) U2 (3) U4 (4) U5 The snRNA that base pairs with […]
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Role of U2 snRNA in Splicing and Branch Point Recognition
22. An intron in a yeast reporter gene carries a mutation in the splice site branch point (UACUAAC to UACA*AAC). To suppress the mutation, a library of point mutants of […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
mRNA Export Required ATP | Unspliced mRNA Retention | Dbp5 Role as RNA helicase
21. Which one of the following statements about mRNA export from the nucleus is INCORRECT? (1) Cellular pre-rnRNAs with introns are generally not exported out of nucleus (2) Dbp5 is […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Nuclear Retention and Export of Pre-mRNAs with Splice Site Mutations
20. In order to ensure that only fully processed mature mRNAs are allowed to be exported to cytosol, pre- mRNAs associated with snRNPs are retained in the nucleus. To demonstrate […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Nucleus Export Spliced RNA associated with the poly A binding and cap binding complex
19. Which one of the following mRNAs is most likely to be exported out of the nucleus? (1) Spliced RNA associated with the poly A binding and cap binding complex. […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Pre-mRNA Splicing | snRNPs and the Spliceosome
18. Pre-mRNAs are rapidly bound by snRNPs which carry out dual steps of splicing that removes the intron and joins the upstream and downstream exons. The following statements describe some […]
