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11. An in vitro translation system capable of incorporating ~8 amino acids was programmed to translate a single mRNA that codes for an alanine-rich (35% alanine with uniform distribution of […]

10. A reporter cell line with stably integrated retroviral promoter-luciferase construct was transfected with an expression vector for a cellular protein. The protein seems to regulate the activation of retroviral […]

9. A researcher wanted to identify the enhancer sequences of a newly discovered gene. Shown below are the relevant regions of some of the reporter constructs the researcher designed to […]

The data from an S1 nuclease mapping experiment for a transcript mfg1 using a 5′-end labelled probe are shown below. Following interpretations were made: A. The liver RNA is 500 […]

7. Run off transcription assays were performed to establish the specificity of three novel sigma factors for their promoters. Result of the experiments are shown below: Following inferences were made […]

6. A researcher was working with three proteins, A, B and C which may have potential roles in gene expression. In order to validate the hypothesis, EMSA (electrophoretic mobility shift […]

Deletion analysis of a promoter region of a gene was carried out to identify the regulatory elements in it. In the figure below, the filled boxes denote the areas of […]

In order to identify the regulatory regions of a novel promoter sequence shown above, four 150 bp deletion constructs were made in a luciferase reporter system as indicated above in […]

3. A promoter deletion study was done in order to determine the binding sites for a transcription factor on the promoter, which is activated on treatment with the drug ‘X’. […]

Which of the following methods can be used to study protein-protein interactions? (1) Co-immunoprecipitation (2) Peptide mapping (3) Radiolabeling (4) Enzyme linked immunoassay  Introduction Protein-protein interactions (PPIs) are fundamental to […]