10 Two E.coli Culture A and B are taken. Culture A was earlier grown in the presence of optimum concentration of gratuitous inducer IPTG. Both the cultures are now used […]
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CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
How E. coli Distinguishes Initiating and Internal Methionine Codons
During protein synthesis in E. coli the initiating methionine (AUG) and the internal methionine (AUG) codons of a given mRNA are distinguished by (1) fmet-tRNAf and met-tRNAm respectively (2) fmet-tRNAf , IF-2 versus […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Diauxic Growth in E. coli: How Glucose and Lactose Shape the Bacterial Growth Curve
9. A culture medium contains two carbon sources, one is preferred carbon source (glucose) and the second is a non-preferred source (lactose). Which one below is correct regarding the nature […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Which RNA Carries Peptidyl Transferase Activity
Which one Of the following RNAs possesses the peptidyl transferase activity? (1) tRNA (2) 2.5S rRNA (3) 16S rRNA (4) 23S rRNA Introduction […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Glucose Addition and Lac Operon Expression
8. E. coli cells are growing in a medium containing lactose but no glucose. Indicate the expression status of the lac operon if glucose is added to the culture. (1) […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Which Statement About Eukaryotic Translation Is NOT True?
Which one of the following statements about eukaryotic translation is NOT true? (1) ribosome binding site on mRNA is called Kozak consensus sequences. (2) initiatortRNA is tRNAf-met (3) initiator amino […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
How β-Galactosidase Expression in E. coli Changes After Glucose Depletion in a Galactose Medium
7. E. coli is being grown in a medium containing both glucose and galactose. On depletion of glucose, expression of β-galactosidase will (1) remain unchanged (2) increase (3) decrease (4) […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Prokaryotic Translation Initiation: The Role of IF-2 and Initiator t-RNA
In prokaryotes IF-2 binds to (1) Initiator t-RNA and GTP (2) Amino acyl t-RNA and ATP (3) Ribosome and m-RNA (4) m-RNA and 30 S subunit of ribosome Introduction Protein […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Lac Operon Regulation By High cAMP, Allolactose and CAP Activity
6. The lac operon in E. coli. is controlled by both the lac repressor and the catabolite activation protein CAP. In an in vivo experiment with lac operon, the following […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Eukaryotic Translation Initiation: Beyond eIF2 and Met-tRNA
For translation initiation process besides elF2, Met-t- RNA eukaryotic 80-S ribosome also requires (1) GTP (2) ATP (3) CTP (4) UTP Introduction Translation initiation in eukaryotes is a highly regulated […]
