It has been observed that in 5-10% of the eukaryotic mRNAs with multiple AUGs, the first AUG is not the initiation site, In such cases, the ribosome skips over one […]
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CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Growth Curve of lacI Mutant E. coli in Glucose and Lactose
14. The following figure represent the growth curve of wild type E.coli grown in a medium containing both glucose and lactose. In E.coli, the catabolite repression of lactose Operon by […]
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Circular mRNA and Protein Synthesis: Mechanisms Behind Rapid Translation in Eukaryotic Cells
presence of Circular mRNAs a specific protein in an eukaryotic cell reflects a rapid rate of synthesis Of that protein. following mechanisms are suggested: A) Eif-4G and PABP promote this […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
During Translation Termination the Release Factor Binds to Stop Codon
During translation when release factor binds to stop codon on m-RNA in A-site of ribosome, the synthesized translation peptide transferred to (1) t-RNA (2) Water (3) H+(4) Amino acids Introduction […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
β-Galactosidase Activity in Merodiploid E. coli
13. A merodiploid strain of E. coli with the genotype F+OCZ–Y+/ O+Z+Y+was constructed. The activity of β-galactosidase enzyme was measured in this strain upon following treatments. (A) no induction (B) […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
The Critical Step During Elongation of Translation is Translocation of the Ribosome
The most critial step during elongation for correct proteins synthesis is (1) Binding of ribosome to m.RNA (2) Formation Initiation complex (3) Aminoacylation of t-RNA (4) Translocation Of ribosome Introduction […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Catabolite Repression in Bacteria
A bacterial stra•n can use carbohydrates and hydrocarbons as growth substrates. The strain uses glucose following a minimal lag period after inoculation, regardless of the other carbohydrates and hydrocarbons in […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
What Is the Kozak Sequence
Which the following names is appropriate for the sequence 5′-G/ANNAUG -3′ in a mammalian mRNA? (1) Shine-Dalgarno sequence (2) Kozak sequence (3) Internal ribosome entry Sites (4) Translation termination site […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Budding Yeast Show Two Lag Phases When Grown on High Glucose
11. When budding yeast (a facultative anaerobe) is grown for a few days in medium containing high glucose it shows a growth pattern with two lag phases (see figure below). […]
CSIR NET Life Science Previous Year Questions and Solution on Molecular Biology
Peptidyl Transferase Activity in Prokaryotic Protein Synthesis
During protein synthesis in prokaryotes, the peptidyl transferase activity required for peptide bond is due to (1) ribosomal protein L26. (2) 16S ribosomal RNA. (3) 23S ribosomal RNA. (4) aminoacyltRNA. […]
