Which of the following is NOT a post-translational modification in a mammalian system? (1) palmitoylation (2) glycosylation (3) peptidylation (4) phosphorylation Introduction to Post-Translational Modifications Post-translational modifications (PTMs) are chemical […]
23. In an experimental approach regulatory sequences of trp operon were fused with structural genes of lac operon. Under what condition there would be expression Of β-galactosidase? (1) Low lactose […]
Which one of following modification of proteins is co translational? (1) Palmitoylation (2) Myristoylation (3) Famesylation (4) addition of cholesterol Introduction to Protein Modification Proteins undergo a variety of modifications […]
A hypothetical operon involved in the synthesis of an amino acid ‘X’ is ‘ON’ (transcribing) in the presence of low levels of ‘X’ and ‘Off’ (not transcribing) in presence of […]
In m-RNA immature termination occurs due to stop codon UAA. Which of the following transition mutation will lead to its reversal? (1) Change at first U (2) Change at first […]
During translation in prokaryotes, when ribosomes reach the termination codon, the termination codon is recognized by the class I release factors (RF1 or RF2) leading to the release of the […]
The genetic code has 61 codons for amino acids and termination codons. How protein synthesis is terminated? (1) There are specific tRNAs for stop codons (2) Release factors mediate termination […]
During elongation step or protein synthesis, translocation moves the mRNA and the peptidyl t-RNA by one codon through the ribosome. Translocation in E.coli involves GTP and EF-G. However, in vitro […]
A protein has 4 equally spaced trypsin sensitive sites which results in peptide fragments A1, A2, A3, A4 and A5 upon digestion with trypsin. Peptides A2 and As, represents N-terminal […]
19. Which one of the following statements relating to the mechanism of color development in response to LacZ expression in Escherichia coli is INCORRECT? (1) E. coli growth on LB […]
