Following statements were made about mitochondria: (A) The D loop of the mitochondrial genome is required for replication, but not for the regulation of transcription. (B) The L strand of […]
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CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Key Features of the Human Mitochondrial Genome: Replication, Transcription, and Structure
86. Following statements were made about human mitochondrial genome: A. The replication of both the H and L strands is unidirectional and begins at specific origins B. Majority of the […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Understanding the High Copy Number of pUC18: Mutation in RNAII and Absence of Rop Gene
The ColE1 plasmid has a low to medium copy number. However, pUC18, which is also a ColE1- based plasmid, has a high copy number because: (1) It has a mutation […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How Mutations in RepA Dimerization Domain Convert Stringent Plasmid Replication to Relaxed Mode
Plasmid copy number achieved by plasmid-encoded control elements that regulate the initiation of the replication step. For example in stringent plasmid protein Rep A dimerize and binds to origin of […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Retroviral Reverse Transcriptase: The RNA-Dependent DNA Polymerase Essential for Viral Replication
Reverse transcriptase of retero virus is- (1) DNA dependent DNA polymerase (2) DNA dependent RNA polymerase (3) RNA dependent DNA polymerase (4) RNA dependent RNA polymerase What Is Retroviral […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Understanding the Ribonuclease Activity of Reverse Transcriptase: Degradation of RNA Strand in RNA/DNA Hybrids
Reverse transcriptase has both ribonuclease and polymerase activities. Ribonuclease activity is required for (1)the synthesis of new RNA strand (2)the degradation RNA strand (3)the synthesis of new DNA strand (4)the […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Insights into Plasmid Curing and Segregation: Random Distribution vs Active Partitioning in Bacteria
81. A bacterial population has a plasmid with copy number ‘n’. It was observed that on an average in one out of 2(n-1) cell divisions. there was spontaneous plasmid curing. […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
How RNase H Influences the Mobility of Newly Synthesized DNA Fragments in Urea-PAGE Analysis
In an in vitro experiment using radio-labeled nucleotides, a researcher is trying to analyze the possible products or DNA replication by resolving the products using urea-polyacrylamide gel electrophoresis. In one […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Understanding DNA Supercoiling Removal During Replication: Roles of Helicase and Topoisomerases
Following statements have been made about removal of supercoiling produced by DNA unwinding at the replication fork: Accumulation of supercoils is the result of DNA helicase activity during unwinding of […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Topoisomerases as Targets for Anticancer Drugs: Clarifying Common Misconceptions
As topoisomerases play an important role during replication, a large number of anticancer drugs have been developed that inhibit the activity of these enzymes. Which of the following statements is […]
