The Temperature of Melting (Tm) is defined as the temperature at which 50% of double stranded DNA is changed to single-standard DNA. Tm of DNA does not depends on (1) […]
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CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Calculate DNA Denaturation Enthalpy (ΔH) at 37°C from Values at Higher Temperatures
It has been observed that for the DNA double helix melting, the value of ΔH (enthalpy change of denaturation) are 80 and 90 kcal/mole at 700 and 800, respectively. Assuming […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Estimate Protein Stability Thermodynamics Using Equilibrium Constant and Temperature
Protein stability is represented as Prior to development of sensitive calorimeters, thermodynamic parameters of processes were determined by following equation ΔHO and ΔSO are standard changes m enthalpy and entropy, […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How to Calculate Gibbs Free Energy Change (ΔG) of DNA Denaturation at 37°C from DSC Data
Differential scanning calorimetric study of calf thymus DNA was carried out to measure midpoint of thermal denaturation (Tm). ΔHm (enthalpy change at Tm) and ΔCP (constant-pressure heat capacity change). It […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding SDS-Induced Protein Denaturation: Effects on Secondary and Tertiary Structures
Sodium dodecyl sulphate (SDS) denatures protein by altering their (1) Primary structure only (2) Primary and secondary structure (3) Secondary and tertiary structure (4) Primary, secondary and tertiary structure […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Which Guanidinium Salt Is the Most Effective Protein Denaturant? Understanding the Role of Counterions
Most effective protein denaturant form of guanidinium when used in equimolar concentration is (1) Iodide (2) Chloride (3) Bromide (4) Sulphate Introduction Guanidinium salts are widely used chemical denaturants […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
How Salt Concentration Influences Protein Stability: Increased Hydrophobic Interaction and Reduced Electrostatic Repulsion Raise Tm
A protein in 100 mM KCl solution was heated and the observed Tm (midpoint of unfolding) was 600C. When the same protein solution in 500 mM KCl was heated, the […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
Understanding Protein Denaturation by Organic Solvents: Hydrophobic Interactions Are Mainly Affected
Globular protein when treated with organic solvent get denatured. The main interaction which is affected on treatment with organic solvent is (1) Hydrogen bonds (2) Covalent bonds (3) Ionic interactions […]
CSIR NET Life Science Previous Year Questions and Solution on Biochemistry
What Determines the Shape of Protein Stability Curves? The Crucial Role of Hydrophobic Interactions
Values of Tm (midpoint of denaturation), ΔHm (enthalpy change at Tm) and ΔCP (constant-pressure heat capacity change) of a protein are measured in a differential scanning calorimeter. ΔGD(T), the Gibbs […]
CSIR NET Life Science Previous Year Questions and Solution on DNA REPLICATION
Understanding the Replication Process of ΦX174: From Single-Stranded DNA to Progeny Phages
Key Points About ΦX174 Replication 88. Reproduction of Φ x 174, a single stranded DNA phage involves several steps. A few statements are given below to explain the mechanism. […]
